1sau: Difference between revisions

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[[Image:1sau.gif|left|200px]]<br /><applet load="1sau" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1sau, resolution 1.12&Aring;" />
'''The Gamma subunit of the dissimilatory sulfite reductase (DsrC) from Archaeoglobus fulgidus at 1.1 A resolution'''<br />


==Overview==
==The Gamma subunit of the dissimilatory sulfite reductase (DsrC) from Archaeoglobus fulgidus at 1.1 A resolution==
The structure of the 115 amino-acid residue protein DsvC was determined based on the anomalous scattering provided by the five S atoms present in the structure. By collecting the diffraction data at a wavelength of 1.9 A, the anomalous signal provided by the S atoms was enhanced. However, significant radiation damage occurred during the course of the experiment, which led to differences between different parts of the data set. Only by dividing the total data set into five data sets was it possible to obtain phases; these could then be successfully extended to allow structure determination by the automated model-building program ARP/wARP. A computational correction for the radiation damage was found to significantly improve the success rate in determining the heavy-atom substructure and to improve phasing and refinement statistics.
<StructureSection load='1sau' size='340' side='right'caption='[[1sau]], [[Resolution|resolution]] 1.12&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1sau]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus_DSM_4304 Archaeoglobus fulgidus DSM 4304]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SAU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SAU FirstGlance]. <br>
1SAU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus_dsm_4304 Archaeoglobus fulgidus dsm 4304]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SAU OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.12&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sau FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sau OCA], [https://pdbe.org/1sau PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sau RCSB], [https://www.ebi.ac.uk/pdbsum/1sau PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sau ProSAT]</span></td></tr>
==Reference==
</table>
Determination of a novel structure by a combination of long-wavelength sulfur phasing and radiation-damage-induced phasing., Weiss MS, Mander G, Hedderich R, Diederichs K, Ermler U, Warkentin E, Acta Crystallogr D Biol Crystallogr. 2004 Apr;60(Pt 4):686-95. Epub 2004, Mar 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15039557 15039557]
== Function ==
[[Category: Archaeoglobus fulgidus dsm 4304]]
[https://www.uniprot.org/uniprot/O28055_ARCFU O28055_ARCFU]
[[Category: Single protein]]
== Evolutionary Conservation ==
[[Category: Ermler, U.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Hedderich, R.]]
Check<jmol>
[[Category: Kahnt, J.]]
  <jmolCheckbox>
[[Category: Mander, G J.]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sa/1sau_consurf.spt"</scriptWhenChecked>
[[Category: Warkentin, E.]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: Weiss, M S.]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: orthogonal helical bundle]]
  </jmolCheckbox>
 
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sau ConSurf].
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:59:38 2008''
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Archaeoglobus fulgidus DSM 4304]]
[[Category: Large Structures]]
[[Category: Ermler U]]
[[Category: Hedderich R]]
[[Category: Kahnt J]]
[[Category: Mander GJ]]
[[Category: Warkentin E]]
[[Category: Weiss MS]]
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