4hz1: Difference between revisions

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[[Image:4hz1.jpg|left|200px]]


{{STRUCTURE_4hz1| PDB=4hz1 | SCENE= }}
==Crystal Structure of Pseudomonas aeruginosa azurin with iron(II) at the copper-binding site.==
<StructureSection load='4hz1' size='340' side='right'caption='[[4hz1]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4hz1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HZ1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HZ1 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hz1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hz1 OCA], [https://pdbe.org/4hz1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hz1 RCSB], [https://www.ebi.ac.uk/pdbsum/4hz1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hz1 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/AZUR_PSEAE AZUR_PSEAE] Transfers electrons from cytochrome c551 to cytochrome oxidase.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The apoprotein of Pseudomonas aeruginosa azurin binds iron(II) to give a 1:1 complex, which has been characterized by electronic absorption, Mossbauer, and NMR spectroscopies, as well as X-ray crystallography and quantum-chemical computations. Despite potential competition by water and other coordinating residues, iron(II) binds tightly to the low-coordinate site. The iron(II) complex does not react with chemical redox agents to undergo oxidation or reduction. Spectroscopically calibrated quantum-chemical computations show that the complex has high-spin iron(II) in a pseudotetrahedral coordination environment, which features interactions with side chains of two histidines and a cysteine as well as the C horizontal lineO of Gly45. In the (5)A(1) ground state, the d(z(2)) orbital is doubly occupied. Mutation of Met121 to Ala leaves the metal site in a similar environment but creates a pocket for reversible binding of small anions to the iron(II) center. Specifically, azide forms a high-spin iron(II) complex and cyanide forms a low-spin iron(II) complex.


===Crystal Structure of Pseudomonas aeruginosa azurin with iron(II) at the copper-binding site.===
Azurin as a Protein Scaffold for a Low-coordinate Nonheme Iron Site with a Small-molecule Binding Pocket.,McLaughlin MP, Retegan M, Bill E, Payne TM, Shafaat HS, Pena S, Sudhamsu J, Ensign AA, Crane BR, Neese F, Holland PL J Am Chem Soc. 2012 Dec 5;134(48):19746-57. doi: 10.1021/ja308346b. Epub 2012 Nov, 20. PMID:23167247<ref>PMID:23167247</ref>


{{ABSTRACT_PUBMED_23167247}}
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4hz1" style="background-color:#fffaf0;"></div>


==About this Structure==
==See Also==
[[4hz1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HZ1 OCA].
*[[Azurin 3D structures|Azurin 3D structures]]
[[Category: Pseudomonas aeruginosa pao1]]
== References ==
[[Category: Bill, E.]]
<references/>
[[Category: Crane, B R.]]
__TOC__
[[Category: Ensign, A A.]]
</StructureSection>
[[Category: Holland, P L.]]
[[Category: Large Structures]]
[[Category: McLaughlin, M P.]]
[[Category: Pseudomonas aeruginosa PAO1]]
[[Category: Neese, F.]]
[[Category: Bill E]]
[[Category: Payne, T M.]]
[[Category: Crane BR]]
[[Category: Pea, S.]]
[[Category: Ensign AA]]
[[Category: Retegan, M.]]
[[Category: Holland PL]]
[[Category: Shafaat, H S.]]
[[Category: McLaughlin MP]]
[[Category: Sudhamsu, J.]]
[[Category: Neese F]]
[[Category: Arsenite]]
[[Category: Payne TM]]
[[Category: Copper binding site]]
[[Category: Pea S]]
[[Category: Electron transport]]
[[Category: Retegan M]]
[[Category: Reductase]]
[[Category: Shafaat HS]]
[[Category: Sudhamsu J]]
Retrieved from "https://proteopedia.org/4hz1"