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[[Image:1syy.gif|left|200px]]<br /><applet load="1syy" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1syy, resolution 1.70&Aring;" />
'''Crystal structure of the R2 subunit of ribonucleotide reductase from Chlamydia trachomatis'''<br />


==Overview==
==Crystal structure of the R2 subunit of ribonucleotide reductase from Chlamydia trachomatis==
Ribonucleotide reductase (RNR) synthesizes the deoxyribonucleotides for DNA synthesis. The R2 protein of normal class I ribonucleotide reductases contains a diiron site that produces a stable tyrosyl free radical, essential for enzymatic activity. Structural and electron paramagnetic resonance studies of R2 from Chlamydia trachomatis reveal a protein lacking a tyrosyl radical site. Instead, the protein yields an iron-coupled radical upon reconstitution. The coordinating structure of the diiron site is similar to that of diiron oxidases/monoxygenases and supports a role for this radical in the RNR mechanism. The specific ligand pattern in the C. trachomatis R2 metal site characterizes a new group of R2 proteins that so far has been found in eight organisms, three of which are human pathogens.
<StructureSection load='1syy' size='340' side='right'caption='[[1syy]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1syy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydia_trachomatis Chlamydia trachomatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SYY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SYY FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=PB:LEAD+(II)+ION'>PB</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1syy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1syy OCA], [https://pdbe.org/1syy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1syy RCSB], [https://www.ebi.ac.uk/pdbsum/1syy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1syy ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RIR2_CHLTR RIR2_CHLTR] Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sy/1syy_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1syy ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1SYY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Chlamydia_trachomatis Chlamydia trachomatis] with <scene name='pdbligand=FE:'>FE</scene> and <scene name='pdbligand=PB:'>PB</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ribonucleoside-diphosphate_reductase Ribonucleoside-diphosphate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.17.4.1 1.17.4.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SYY OCA].
*[[Ribonucleotide reductase 3D structures|Ribonucleotide reductase 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
The radical site in chlamydial ribonucleotide reductase defines a new R2 subclass., Hogbom M, Stenmark P, Voevodskaya N, McClarty G, Graslund A, Nordlund P, Science. 2004 Jul 9;305(5681):245-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15247479 15247479]
[[Category: Chlamydia trachomatis]]
[[Category: Chlamydia trachomatis]]
[[Category: Ribonucleoside-diphosphate reductase]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Graslund A]]
[[Category: Graslund, A.]]
[[Category: Hogbom M]]
[[Category: Hogbom, M.]]
[[Category: McClarty G]]
[[Category: McClarty, G.]]
[[Category: Nordlund P]]
[[Category: Nordlund, P.]]
[[Category: Stenmark P]]
[[Category: Stenmark, P.]]
[[Category: Voevodskaya N]]
[[Category: Voevodskaya, N.]]
[[Category: FE]]
[[Category: PB]]
[[Category: diiron; oxygen activation; iron coupled radical; immune evasion]]
 
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Retrieved from "https://proteopedia.org/1syy"