1qfd: Difference between revisions
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==NMR SOLUTION STRUCTURE OF ALPHA-AMYLASE INHIBITOR (AAI)== | |||
<StructureSection load='1qfd' size='340' side='right'caption='[[1qfd]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1qfd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Amaranthus_hypochondriacus Amaranthus hypochondriacus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QFD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QFD FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 10 models</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qfd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qfd OCA], [https://pdbe.org/1qfd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qfd RCSB], [https://www.ebi.ac.uk/pdbsum/1qfd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qfd ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/IAAI_AMAHP IAAI_AMAHP] Alpha-amylase inhibitor. It is active against alpha-amylases from Tribolium castaneum and Prostephanus truncatus larvae. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
alpha-Amylase inhibitor (AAI), a 32-residue miniprotein from the Mexican crop plant amaranth (Amaranthus hypochondriacus), is the smallest known alpha-amylase inhibitor and is specific for insect alpha-amylases (Chagolla-Lopez, A., Blanco-Labra, A., Patthy, A., Sanchez, R., and Pongor, S. (1994) J. Biol. Chem. 269, 23675-23680). Its disulfide topology was confirmed by Edman degradation, and its three-dimensional solution structure was determined by two-dimensional 1H NMR spectroscopy at 500 MHz. Structural constraints (consisting of 348 nuclear Overhauser effect interproton distances, 8 backbone dihedral constraints, and 9 disulfide distance constraints) were used as an input to the X-PLOR program for simulated annealing and energy minimization calculations. The final set of 10 structures had a mean pairwise root mean square deviation of 0.32 A for the backbone atoms and 1.04 A for all heavy atoms. The structure of AAI consists of a short triple-stranded beta-sheet stabilized by three disulfide bonds, forming a typical knottin or inhibitor cystine knot fold found in miniproteins, which binds various macromolecular ligands. When the first intercystine segment of AAI (sequence IPKWNR) was inserted into a homologous position of the spider toxin Huwentoxin I, the resulting chimera showed a significant inhibitory activity, suggesting that this segment takes part in enzyme binding. | |||
Solution structure of the major alpha-amylase inhibitor of the crop plant amaranth.,Lu S, Deng P, Liu X, Luo J, Han R, Gu X, Liang S, Wang X, Li F, Lozanov V, Patthy A, Pongor S J Biol Chem. 1999 Jul 16;274(29):20473-8. PMID:10400675<ref>PMID:10400675</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== | <div class="pdbe-citations 1qfd" style="background-color:#fffaf0;"></div> | ||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Amaranthus hypochondriacus]] | [[Category: Amaranthus hypochondriacus]] | ||
[[Category: Deng | [[Category: Large Structures]] | ||
[[Category: Feng | [[Category: Deng P]] | ||
[[Category: Gu | [[Category: Feng L]] | ||
[[Category: Han | [[Category: Gu X]] | ||
[[Category: Liang | [[Category: Han R]] | ||
[[Category: Liu | [[Category: Liang S]] | ||
[[Category: Lozanov | [[Category: Liu X]] | ||
[[Category: Lu | [[Category: Lozanov V]] | ||
[[Category: Luo | [[Category: Lu S]] | ||
[[Category: Patthy | [[Category: Luo J]] | ||
[[Category: Pongor | [[Category: Patthy A]] | ||
[[Category: Wang | [[Category: Pongor S]] | ||
[[Category: Wang X]] | |||
Latest revision as of 04:49, 17 October 2024
NMR SOLUTION STRUCTURE OF ALPHA-AMYLASE INHIBITOR (AAI)
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