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| {{STRUCTURE_1gx1| PDB=1gx1 | SIZE=400| SCENE= |right|CAPTION=Se-Met MECDP synthase trimer complex with CDP, sulfate, Mn+2 (purple) and Zn+2 (grey) ion, [[1gx1]] }}
| | <StructureSection load='1gx1' size='450' side='right' scene='52/526157/Cv/1' caption='Se-Met MECDP synthase trimer complex with CDP, sulfate, Mn+2 (purple) and Zn+2 (grey) ion, [[1gx1]]'> |
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| '''MECDP synthase''' (MECPS) participates in mevalonate-independent steroid biosynthesis. It converts 2-phospho-4-(cytidine 5’-diphospho)-2-C-methyl-D-erythritol (2P-CDME) to 2-C-methyl-D-erythritol 2, 4-cyclodiphosphate (MECP) and CMP. MECPS contains metal binding sites which are required for catalysis. MECPS is a potential antimalarial drug target. | | == Function == |
| | '''MECDP synthase''' or '''2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase''' (MECPS) participates in mevalonate-independent steroid biosynthesis. It converts 2-phospho-4-(cytidine 5’-diphospho)-2-C-methyl-D-erythritol (2P-CDME) to 2-C-methyl-D-erythritol 2, 4-cyclodiphosphate (MECP) and CMP. MECPS contains metal binding sites which are required for catalysis<ref>PMID:11786530</ref>. |
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| ===3D structures of MECDP synthase=== | | == Relevance == |
| | MECPS is a potential antimalarial drug target<ref>PMID:12499535</ref>. |
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| [[1knk]] - EcMECPS + Mn – ''Escherichia coli''<br />
| | == Structural highlights == |
| [[1u3p]] - EcMECPS + Zn<br />
| | <scene name='52/526157/Cv/7'>MECPS active site is located in a cleft between 2 subunits</scene> and contains the <scene name='52/526157/Cv/8'>metal ions</scene><ref>PMID:11997478</ref>. Water molecules are shown as red spheres. |
| [[3f0d]] - BpMECPS + Zn – ''Burkholderia pseudomallei''<br />
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| [[3f0e]] - BpMECPS + Mg + Zn<br />
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| [[1jn1]] - HiMECPS + Co – ''Haemophilus influenzae'' <br />
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| [[1vh8]] - HiMECPS <br />
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| [[1vha]] - HiMECPS + Mn<br />
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| [[1iv1]] - TtMECPS – ''Thermus thermophilus''<br />
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| [[3f6m]] - YpMECPS – ''Yersinia pestis''<br />
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| [[1iv3]] - TtMECPS (mutant) + Mg<br />
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| [[3ghz]] - MECPS + Mg – ''Salmonella typhimurium''<br />
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| [[3re3]] - MECPS – ''Francisella tularensis''
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| ==MECPS binary complex== | | ==3D structures of MECDP synthase== |
| | [[MECDP synthase 3D structures]] |
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| [[1gx1]] - EcMECPS + CDP + Mn + Zn<br />
| | </StructureSection> |
| [[1u3l]] - EcMECPS + CDP + Mg + Zn<br />
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| [[3f0f]] - BpMECPS + CMP + Mg + Zn<br />
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| [[3ike]] - BpMECPS + cytosine + Mg + Zn<br />
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| [[3kix]], [[3ke1]] - BpMECPS + cytosine derivative + Zn<br />
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| [[3ikf]], [[3k14]], [[3q8h]] - BpMECPS + imidazo-thiazol derivative + Zn<br />
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| [[3f0g]], [[3ieq]] - BpMECPS + CMP + Zn<br />
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| [[2uzh]] - MECPS + CDP + Zn – ''Mycobacterium smegmatis''<br />
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| [[3fpi]] - YpMECPS + CTP + Mg<br />
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| [[1iv2]] - TtMECPS (mutant) + CDP + Mg<br />
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| [[1iv4]] - TtMECPS (mutant) + CMP + Mg<br />
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| [[1h47]] - EcMECPS + CMP + Zn<br />
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| [[3jvh]] - BpMECPS + pyrimidine derivative + Zn<br />
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| [[1u40]] - EcMECPS + CDME + Zn<br />
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| [[1u43]] - EcMECPS + 2P-CDME<br />
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| [[1t0a]] - MECPS + farnesyl phosphate + Co + Zn - ''Shewanella oneidensis''<br />
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| [[2pmp]] - MECPS + CMP + Zn – ''Arabidopsis thaliana''
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| ==MECPS ternary complex== | | == References == |
| | | <references/> |
| [[3iew]] - BpMECPS + CDP + CTP + Zn<br />
| | [[Category:Topic Page]] |
| [[1jy8]] – EcMECPS + MECP + CMP + Zn<br />
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| [[1knj]] - EcMECPS + MECP + CMP + Mn<br />
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| [[3mbm]] - BpMECPS + imidazo-thiazol derivative +cytosine + Zn<br />
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| [[3p10]] - BpMECPS + pyrimidine derivative + cytidine + Zn<br />
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| [[3t80]] - MECPS + pyrimidine derivative + cytidine + Zn – ''Salmonella enterica''<br />
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| [[1yqn]] – EcMECPS (mutant) + CMP + geranyl diphosphate + Mn + Zn<br />
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| [[2amt]] - EcMECPS + CMP derivative + geranyl diphosphate + Zn<br /> | |
| [[3elc]], [[3eor]], [[3ern]], [[3fba]] - EcMECPS + pyrimidine derivative + geranyl diphosphate + Zn<br />
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| [[3esj]] - EcMECPS + pyrimidine derivative + geranyl diphosphate + Mg + Zn<br />
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| [[2gzl]] - EcMECPS + inhibitor + geranyl diphosphate + Zn<br />
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| ==MECPS quaternary complex==
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| [[3p0z]], [[3qhd]] - BpMECPS + imidazo derivative + pyrimidine derivative + cytidine + Zn<br />
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| [[1h48]] - EcMECPS + MECP + CMP + geranyl diphosphate + Mn + Zn<br />
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