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[[Image:1tks.gif|left|200px]]<br /><applet load="1tks" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1tks, resolution 1.6&Aring;" />
'''Crystal structure of 3,4-Dihydroxy-2-butanone 4-phosphate Synthase of Candida albicans'''<br />


==Overview==
==Crystal structure of 3,4-Dihydroxy-2-butanone 4-phosphate Synthase of Candida albicans==
<StructureSection load='1tks' size='340' side='right'caption='[[1tks]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1tks]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_albicans_SC5314 Candida albicans SC5314]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TKS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TKS FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tks OCA], [https://pdbe.org/1tks PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tks RCSB], [https://www.ebi.ac.uk/pdbsum/1tks PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tks ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RIB3_CANAL RIB3_CANAL] Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate (By similarity).[HAMAP-Rule:MF_00180]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tk/1tks_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tks ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
A synthetic gene specifying a putative 3,4-dihydroxy-2-butanone 4-phosphate synthase of Candida albicans directed the synthesis of a 22.5 kDa peptide in a recombinant Escherichia coli strain. The recombinant protein was purified to apparent homogeneity by two chromatographic steps and was shown to catalyze the formation of L-3,4-dihydroxy-2-butanone 4-phosphate from ribulose 5-phosphate at a rate of 332 nmol mg(-1) min(-1). Hydrodynamic studies indicated a native molecular mass of 41 kDa in line with a homodimer structure. The protein was crystallized in its apoform. Soaking yielded crystals in complex with the substrate ribulose 5-phosphate. The structures were solved at resolutions of 1.6 and 1.7 angstroms, respectively, using 3,4-dihydroxy-2-butanone 4-phosphate synthase of E. coli for molecular replacement. Structural comparison with the orthologs of Magnaporthe grisea and Methanococcus jannaschii revealed a hitherto unknown conformation of the essential acidic active-site loop.
A synthetic gene specifying a putative 3,4-dihydroxy-2-butanone 4-phosphate synthase of Candida albicans directed the synthesis of a 22.5 kDa peptide in a recombinant Escherichia coli strain. The recombinant protein was purified to apparent homogeneity by two chromatographic steps and was shown to catalyze the formation of L-3,4-dihydroxy-2-butanone 4-phosphate from ribulose 5-phosphate at a rate of 332 nmol mg(-1) min(-1). Hydrodynamic studies indicated a native molecular mass of 41 kDa in line with a homodimer structure. The protein was crystallized in its apoform. Soaking yielded crystals in complex with the substrate ribulose 5-phosphate. The structures were solved at resolutions of 1.6 and 1.7 angstroms, respectively, using 3,4-dihydroxy-2-butanone 4-phosphate synthase of E. coli for molecular replacement. Structural comparison with the orthologs of Magnaporthe grisea and Methanococcus jannaschii revealed a hitherto unknown conformation of the essential acidic active-site loop.


==About this Structure==
Potential anti-infective targets in pathogenic yeasts: structure and properties of 3,4-dihydroxy-2-butanone 4-phosphate synthase of Candida albicans.,Echt S, Bauer S, Steinbacher S, Huber R, Bacher A, Fischer M J Mol Biol. 2004 Aug 20;341(4):1085-96. PMID:15328619<ref>PMID:15328619</ref>
1TKS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Candida_albicans_sc5314 Candida albicans sc5314]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TKS OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Potential anti-infective targets in pathogenic yeasts: structure and properties of 3,4-dihydroxy-2-butanone 4-phosphate synthase of Candida albicans., Echt S, Bauer S, Steinbacher S, Huber R, Bacher A, Fischer M, J Mol Biol. 2004 Aug 20;341(4):1085-96. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15328619 15328619]
</div>
[[Category: Candida albicans sc5314]]
<div class="pdbe-citations 1tks" style="background-color:#fffaf0;"></div>
[[Category: Single protein]]
== References ==
[[Category: Bacher, A.]]
<references/>
[[Category: Bauer, S.]]
__TOC__
[[Category: Echt, S.]]
</StructureSection>
[[Category: Fischer, M.]]
[[Category: Candida albicans SC5314]]
[[Category: Huber, R.]]
[[Category: Large Structures]]
[[Category: Steinbacher, S.]]
[[Category: Bacher A]]
[[Category: 3]]
[[Category: Bauer S]]
[[Category: 4-dihydroxy-2-butanone 4-phosphate synthase]]
[[Category: Echt S]]
[[Category: candida albicans]]
[[Category: Fischer M]]
[[Category: crystal structure synthetic gene]]
[[Category: Huber R]]
[[Category: riboflavin biosynthesis]]
[[Category: Steinbacher S]]
 
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Retrieved from "https://proteopedia.org/1tks"