1tly: Difference between revisions

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[[Image:1tly.jpg|left|200px]]<br /><applet load="1tly" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1tly, resolution 3.01&Aring;" />
'''Tsx structure'''<br />


==Overview==
==Tsx structure==
Tsx is a nucleoside-specific outer membrane (OM) transporter of Gram-negative bacteria. We present crystal structures of Escherichia coli Tsx in the absence and presence of nucleosides. These structures provide a mechanism for nucleoside transport across the bacterial OM. Tsx forms a monomeric, 12-stranded beta-barrel with a long and narrow channel spanning the outer membrane. The channel, which is shaped like a keyhole, contains several distinct nucleoside-binding sites, two of which are well defined. The base moiety of the nucleoside is located in the narrow part of the keyhole, while the sugar occupies the wider opening. Pairs of aromatic residues and flanking ionizable residues are involved in nucleoside binding. Nucleoside transport presumably occurs by diffusion from one binding site to the next.
<StructureSection load='1tly' size='340' side='right'caption='[[1tly]], [[Resolution|resolution]] 3.01&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1tly]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TLY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TLY FirstGlance]. <br>
1TLY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TLY OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.01&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tly FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tly OCA], [https://pdbe.org/1tly PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tly RCSB], [https://www.ebi.ac.uk/pdbsum/1tly PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tly ProSAT]</span></td></tr>
==Reference==
</table>
Crystal structure of the bacterial nucleoside transporter Tsx., Ye J, van den Berg B, EMBO J. 2004 Aug 18;23(16):3187-95. Epub 2004 Jul 22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15272310 15272310]
== Function ==
[https://www.uniprot.org/uniprot/TSX_ECOLI TSX_ECOLI] Constitutes the receptor for colicin K and phage T6, and functions as substrate-specific channel for nucleosides and deoxynucleosides.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tl/1tly_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tly ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Berg, B van den.]]
[[Category: Ye J]]
[[Category: Ye, J.]]
[[Category: Van den Berg B]]
[[Category: beta barrel]]
[[Category: nucleoside transporter]]
 
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Retrieved from "https://proteopedia.org/1tly"