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[[Image:1gl4.gif|left|200px]]<br />
<applet load="1gl4" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1gl4, resolution 2.00&Aring;" />
'''NIDOGEN-1 G2/PERLECAN IG3 COMPLEX'''<br />


==Overview==
==Nidogen-1 G2/Perlecan IG3 Complex==
Nidogen and perlecan are large multifunctional basement membrane (BM), proteins conserved in all metazoa. Their high-affinity interaction, which, is likely to contribute to BM assembly and function, is mediated by the, central G2 domain in nidogen and the third immunoglobulin (IG)-like domain, in perlecan, IG3. We have solved the crystal structure at 2.0 A resolution, of the mouse nidogen-1 G2-perlecan IG3 complex. Perlecan IG3 belongs to, the I-set of the IG superfamily and binds to the wall of the nidogen-1 G2, beta-barrel using beta-strands C, D and F. Nidogen-1 residues, participating in the extensive interface are highly conserved, whereas the, corresponding binding site on perlecan is more variable. We hypothesize, that a second, as yet unidentified, activity of nidogen overlaps ... [[http://ispc.weizmann.ac.il/pmbin/getpm?11574465 (full description)]]
<StructureSection load='1gl4' size='340' side='right'caption='[[1gl4]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1gl4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GL4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GL4 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gl4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gl4 OCA], [https://pdbe.org/1gl4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gl4 RCSB], [https://www.ebi.ac.uk/pdbsum/1gl4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gl4 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/NID1_MOUSE NID1_MOUSE] Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gl/1gl4_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gl4 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Nidogen and perlecan are large multifunctional basement membrane (BM) proteins conserved in all metazoa. Their high-affinity interaction, which is likely to contribute to BM assembly and function, is mediated by the central G2 domain in nidogen and the third immunoglobulin (IG)-like domain in perlecan, IG3. We have solved the crystal structure at 2.0 A resolution of the mouse nidogen-1 G2-perlecan IG3 complex. Perlecan IG3 belongs to the I-set of the IG superfamily and binds to the wall of the nidogen-1 G2 beta-barrel using beta-strands C, D and F. Nidogen-1 residues participating in the extensive interface are highly conserved, whereas the corresponding binding site on perlecan is more variable. We hypothesize that a second, as yet unidentified, activity of nidogen overlaps with perlecan binding and accounts for the unusually high degree of surface conservation in the G2 domain.


==About this Structure==
Structural basis for the high-affinity interaction of nidogen-1 with immunoglobulin-like domain 3 of perlecan.,Kvansakul M, Hopf M, Ries A, Timpl R, Hohenester E EMBO J. 2001 Oct 1;20(19):5342-6. PMID:11574465<ref>PMID:11574465</ref>
1GL4 is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]] with ZN and EPE as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: EPE. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GL4 OCA]].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Structural basis for the high-affinity interaction of nidogen-1 with immunoglobulin-like domain 3 of perlecan., Kvansakul M, Hopf M, Ries A, Timpl R, Hohenester E, EMBO J. 2001 Oct 1;20(19):5342-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11574465 11574465]
</div>
<div class="pdbe-citations 1gl4" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Protein complex]]
[[Category: Hohenester E]]
[[Category: Hohenester, E.]]
[[Category: Hopf M]]
[[Category: Hopf, M.]]
[[Category: Kvansakul M]]
[[Category: Kvansakul, M.]]
[[Category: Ries A]]
[[Category: Ries, A.]]
[[Category: Timpl R]]
[[Category: Timpl, R.]]
[[Category: EPE]]
[[Category: ZN]]
[[Category: basement membrane]]
[[Category: extracellular matrix]]
[[Category: heparan sulfate]]
[[Category: immunoglobulin-like domain]]
[[Category: proteoglycan]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 15:14:41 2007''
Retrieved from "https://proteopedia.org/1gl4"