1t0w: Difference between revisions

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[[Image:1t0w.png|left|200px]]


{{STRUCTURE_1t0w| PDB=1t0w | SCENE= }}
==25 NMR structures of Truncated Hevein of 32 aa (Hevein-32) complex with N,N,N-triacetylglucosamina==
<StructureSection load='1t0w' size='340' side='right'caption='[[1t0w]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1t0w]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hevea_brasiliensis Hevea brasiliensis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T0W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T0W FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 25 models</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=PRD_900017:triacetyl-beta-chitotriose'>PRD_900017</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t0w OCA], [https://pdbe.org/1t0w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t0w RCSB], [https://www.ebi.ac.uk/pdbsum/1t0w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t0w ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/HEVE_HEVBR HEVE_HEVBR] N-acetyl-D-glucosamine / N-acetyl-D-neuraminic acid binding lectin. Can inhibit fungal growth.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t0/1t0w_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t0w ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
HEV32, a 32-residue, truncated hevein lacking eleven C-terminal amino acids, was synthesized by solid-phase methodology and correctly folded with three cysteine bridge pairs. The affinities of HEV32 for small chitin fragments--in the forms of N,N',N"-triacetylchitotriose ((GlcNAc)3) (millimolar) and N,N',N",N"',N"",N""'-hexaacetylchitohexaose ((GlcNAc)6) (micromolar)--as measured by NMR and fluorescence methods, are comparable with those of native hevein. The HEV32 ligand-binding process is enthalpy driven, while entropy opposes binding. The NMR structure of ligand-bound HEV32 in aqueous solution was determined to be highly similar to the NMR structure of ligand-bound hevein. Solvated molecular-dynamics simulations were performed in order to monitor the changes in side-chain conformation of the binding site of HEV32 and hevein upon interaction with ligands. The calculations suggest that the Trp21 side-chain orientation of HEV32 in the free form differs from that in the bound state; this agrees with fluorescence and thermodynamic data. HEV32 provides a simple molecular model for studying protein-carbohydrate interactions and for understanding the physiological relevance of small native hevein domains lacking C-terminal residues.


===25 NMR structures of Truncated Hevein of 32 aa (Hevein-32) complex with N,N,N-triacetylglucosamina===
NMR and modeling studies of protein-carbohydrate interactions: synthesis, three-dimensional structure, and recognition properties of a minimum hevein domain with binding affinity for chitooligosaccharides.,Aboitiz N, Vila-Perello M, Groves P, Asensio JL, Andreu D, Canada FJ, Jimenez-Barbero J Chembiochem. 2004 Sep 6;5(9):1245-55. PMID:15368576<ref>PMID:15368576</ref>


{{ABSTRACT_PUBMED_15368576}}
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
==About this Structure==
<div class="pdbe-citations 1t0w" style="background-color:#fffaf0;"></div>
[[1t0w]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T0W OCA].
== References ==
 
<references/>
==Reference==
__TOC__
<ref group="xtra">PMID:015368576</ref><references group="xtra"/>
</StructureSection>
[[Category: Aboitiz, N.]]
[[Category: Hevea brasiliensis]]
[[Category: Andreu, D.]]
[[Category: Large Structures]]
[[Category: Asensio, J L.]]
[[Category: Aboitiz N]]
[[Category: Canada, F J.]]
[[Category: Andreu D]]
[[Category: Groves, P.]]
[[Category: Asensio JL]]
[[Category: Jimenez-Barbero, J.]]
[[Category: Canada FJ]]
[[Category: Vila-Perello, M.]]
[[Category: Groves P]]
[[Category: Alpha-helix]]
[[Category: Jimenez-Barbero J]]
[[Category: Anti-parallel beta-sheet]]
[[Category: Vila-Perello M]]
[[Category: Sugar binding protein]]
Retrieved from "https://proteopedia.org/1t0w"