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[[Image:1gsj.gif|left|200px]]<br />
<applet load="1gsj" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1gsj, resolution 1.85&Aring;" />
'''SELENOMETHIONINE SUBSTITUTED N-ACETYL-L-GLUTAMATE KINASE FROM ESCHERICHIA COLI COMPLEXED WITH ITS SUBSTRATE N-ACETYL-L-GLUTAMATE AND ITS SUBSTRATE ANALOG AMPPNP'''<br />


==Overview==
==Selenomethionine substituted N-acetyl-L-glutamate kinase from Escherichia coli complexed with its substrate n-acetyl-L-glutamate and its substrate analog AMPPNP==
N-Acetyl-L-glutamate kinase (NAGK), a member of the amino acid kinase, family, catalyzes the second and frequently controlling step of arginine, synthesis. The Escherichia coli NAGK crystal structure to 1.5 A resolution, reveals a 258-residue subunit homodimer nucleated by a central 16-stranded, molecular open beta sheet sandwiched between alpha helices. In each, subunit, AMPPNP, as an alphabetagamma-phosphate-Mg2+ complex, binds along, the sheet C edge, and N-acetyl-L-glutamate binds near the dyadic axis with, its gamma-COO- aligned at short distance from the gamma-phosphoryl, indicating associative phosphoryl transfer assisted by: (1) Mg2+, complexation; (2) the positive charges on Lys8, Lys217, and on two helix, dipoles; and (3) by hydrogen bonding with the y-phosphate. The structural, ... [[http://ispc.weizmann.ac.il/pmbin/getpm?12005432 (full description)]]
<StructureSection load='1gsj' size='340' side='right'caption='[[1gsj]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1gsj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_BL21(DE3) Escherichia coli BL21(DE3)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GSJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GSJ FirstGlance]. <br>
1GSJ is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with MG, NLG and ANP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Acetylglutamate_kinase Acetylglutamate kinase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.2.8 2.7.2.8]]. Structure known Active Site: NLG. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GSJ OCA]].
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NLG:N-ACETYL-L-GLUTAMATE'>NLG</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gsj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gsj OCA], [https://pdbe.org/1gsj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gsj RCSB], [https://www.ebi.ac.uk/pdbsum/1gsj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gsj ProSAT]</span></td></tr>
Structure of acetylglutamate kinase, a key enzyme for arginine biosynthesis and a prototype for the amino acid kinase enzyme family, during catalysis., Ramon-Maiques S, Marina A, Gil-Ortiz F, Fita I, Rubio V, Structure. 2002 Mar;10(3):329-42. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12005432 12005432]
</table>
[[Category: Acetylglutamate kinase]]
== Function ==
[[Category: Escherichia coli]]
[https://www.uniprot.org/uniprot/ARGB_ECOLI ARGB_ECOLI]  
[[Category: Single protein]]
== Evolutionary Conservation ==
[[Category: Fita, I.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Gil-Ortiz, F.]]
Check<jmol>
[[Category: Marina, A.]]
  <jmolCheckbox>
[[Category: Ramon-Maiques, S.]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gs/1gsj_consurf.spt"</scriptWhenChecked>
[[Category: Rubio, V.]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: ANP]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: MG]]
  </jmolCheckbox>
[[Category: NLG]]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gsj ConSurf].
[[Category: acetylglutamate kinase]]
<div style="clear:both"></div>
[[Category: amino acid kinase]]
__TOC__
[[Category: arginine biosynthesis]]
</StructureSection>
[[Category: carbamate kinase]]
[[Category: Large Structures]]
[[Category: phosphoryl group transfer]]
[[Category: Fita I]]
[[Category: protein crystallography]]
[[Category: Gil-Ortiz F]]
[[Category: selenomethionine]]
[[Category: Marina A]]
 
[[Category: Ramon-Maiques S]]
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 15:18:14 2007''
[[Category: Rubio V]]

Latest revision as of 11:24, 27 March 2024

Selenomethionine substituted N-acetyl-L-glutamate kinase from Escherichia coli complexed with its substrate n-acetyl-L-glutamate and its substrate analog AMPPNP

1gsj, resolution 1.85Å

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