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[[Image:2hh9.png|left|200px]]


{{STRUCTURE_2hh9| PDB=2hh9 | SCENE= }}
==Thiamin pyrophosphokinase from Candida albicans==
<StructureSection load='2hh9' size='340' side='right'caption='[[2hh9]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2hh9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HH9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HH9 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=VIB:3-(4-AMINO-2-METHYL-PYRIMIDIN-5-YLMETHYL)-5-(2-HYDROXY-ETHYL)-4-METHYL-THIAZOL-3-IUM'>VIB</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hh9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hh9 OCA], [https://pdbe.org/2hh9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hh9 RCSB], [https://www.ebi.ac.uk/pdbsum/2hh9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hh9 ProSAT]</span></td></tr>
</table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hh/2hh9_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hh9 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
BACKGROUND: In search of new antifungal targets of potential interest for pharmaceutical companies, we initiated a comparative genomics study to identify the most promising protein-coding genes in fungal genomes. One criterion was the protein sequence conservation between reference pathogenic genomes. A second criterion was that the corresponding gene in Saccharomyces cerevisiae should be essential. Since thiamine pyrophosphate is an essential product involved in a variety of metabolic pathways, proteins responsible for its production satisfied these two criteria. RESULTS: We report the enzymatic characterization and the crystallographic structure of the Candida albicans Thiamine pyrophosphokinase. The protein was co-crystallized with thiamine or thiamine-PNP. CONCLUSION: The presence of an inorganic phosphate in the crystallographic structure opposite the known AMP binding site relative to the thiamine moiety suggests that a second AMP molecule could be accommodated in the C. albicans structure. Together with the crystallographic structures of the enzyme/substrate complexes this suggests the existence of a secondary, less specific, nucleotide binding site in the Candida albicans thiamine pyrophosphokinase which could transiently serve during the release or the binding of ATP. The structures also highlight a conserved Glutamine residue (Q138) which could interact with the ATP alpha-phosphate and act as gatekeeper. Finally, the TPK/Thiamine-PNP complex is consistent with a one step mechanism of pyrophosphorylation.


===Thiamin pyrophosphokinase from Candida albicans===
Structural characterization of CA1462, the Candida albicans thiamine pyrophosphokinase.,Santini S, Monchois V, Mouz N, Sigoillot C, Rousselle T, Claverie JM, Abergel C BMC Struct Biol. 2008 Jul 24;8:33. PMID:18652651<ref>PMID:18652651</ref>


{{ABSTRACT_PUBMED_18652651}}
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
==About this Structure==
<div class="pdbe-citations 2hh9" style="background-color:#fffaf0;"></div>
[[2hh9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HH9 OCA].
== References ==
 
<references/>
==Reference==
__TOC__
<ref group="xtra">PMID:018652651</ref><references group="xtra"/>
</StructureSection>
[[Category: Candida albicans]]
[[Category: Candida albicans]]
[[Category: Thiamine diphosphokinase]]
[[Category: Large Structures]]
[[Category: Abergel, C.]]
[[Category: Abergel C]]
[[Category: Claverie, J M.]]
[[Category: Claverie JM]]
[[Category: IGS-CNRS, France BIGSBacterial targets at.]]
[[Category: Monchois V]]
[[Category: Monchois, V.]]
[[Category: Rousselle T]]
[[Category: Rousselle, T.]]
[[Category: Santini S]]
[[Category: Santini, S.]]
[[Category: Bacterial targets at igs-cnr]]
[[Category: Big]]
[[Category: France]]
[[Category: Structural genomic]]
[[Category: Thiamin]]
[[Category: Thiamin pyrophosphokinase]]
[[Category: Tpk]]
[[Category: Transferase]]
Retrieved from "https://proteopedia.org/2hh9"