2k9b: Difference between revisions

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[[Image:2k9b.png|left|200px]]


{{STRUCTURE_2k9b| PDB=2k9b |  SCENE= }}
==Structure and membrane interactions of the antibiotic peptide dermadistinctin K by multidimensional solution and oriented 15N and 31P solid-state NMR spectroscopy==
<StructureSection load='2k9b' size='340' side='right'caption='[[2k9b]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2k9b]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Phyllomedusa_distincta Phyllomedusa distincta]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K9B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2K9B FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2k9b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k9b OCA], [https://pdbe.org/2k9b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2k9b RCSB], [https://www.ebi.ac.uk/pdbsum/2k9b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2k9b ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DRS1_PHYDS DRS1_PHYDS] Has antibacterial activity against the Gram-positive bacteria S.aureus and E.faecalis, and the Gram-negative bacteria P.aeruginosa and E.coli. Has antiprotozoal activity against T.cruzi. Has antifungal activity against the yeasts C.tropicalis (MIC=10.1 uM), C.guilliermondii (MIC=20.3 uM), C.albicans (MIC=20.3 uM) and C.albicans ATCC 1023 (MIC=10.1 uM). Decreases viability of murine peritoneal cells. Fuses to, and disrupts liposomes.<ref>PMID:10477123</ref> <ref>PMID:12379643</ref> <ref>PMID:17409003</ref> <ref>PMID:17442605</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
DD K, a peptide first isolated from the skin secretion of the Phyllomedusa distincta frog, has been prepared by solid-phase chemical peptide synthesis and its conformation was studied in trifluoroethanol/water as well as in the presence of sodium dodecyl sulfate and dodecylphosphocholine micelles or small unilamellar vesicles. Multidimensional solution NMR spectroscopy indicates an alpha-helical conformation in membrane environments starting at residue 7 and extending to the C-terminal carboxyamide. Furthermore, DD K has been labeled with (15)N at a single alanine position that is located within the helical core region of the sequence. When reconstituted into oriented phosphatidylcholine membranes the resulting (15)N solid-state NMR spectrum shows a well-defined helix alignment parallel to the membrane surface in excellent agreement with the amphipathic character of DD K. Proton-decoupled (31)P solid-state NMR spectroscopy indicates that the peptide creates a high level of disorder at the level of the phospholipid headgroup suggesting that DD K partitions into the bilayer where it severely disrupts membrane packing.


===Structure and membrane interactions of the antibiotic peptide dermadistinctin K by multidimensional solution and oriented 15N and 31P solid-state NMR spectroscopy===
Structure and membrane interactions of the antibiotic peptide dermadistinctin K by multidimensional solution and oriented 15N and 31P solid-state NMR spectroscopy.,Verly RM, de Moraes CM, Resende JM, Aisenbrey C, Bemquerer MP, Pilo-Veloso D, Valente AP, Almeida FC, Bechinger B Biophys J. 2009 Mar 18;96(6):2194-203. PMID:19289046<ref>PMID:19289046</ref>


{{ABSTRACT_PUBMED_19289046}}
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
==About this Structure==
<div class="pdbe-citations 2k9b" style="background-color:#fffaf0;"></div>
[[2k9b]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K9B OCA].
== References ==
 
<references/>
==Reference==
__TOC__
<ref group="xtra">PMID:019289046</ref><references group="xtra"/>
</StructureSection>
[[Category: Aisenbrey, C.]]
[[Category: Large Structures]]
[[Category: Almeida, F C.L.]]
[[Category: Phyllomedusa distincta]]
[[Category: Bechinger, B.]]
[[Category: Aisenbrey C]]
[[Category: Bemquerer, M P.]]
[[Category: Almeida FCL]]
[[Category: Moraes, C M.]]
[[Category: Bechinger B]]
[[Category: Pilo-Veloso, D.]]
[[Category: Bemquerer MP]]
[[Category: Resende, J M.]]
[[Category: Moraes CM]]
[[Category: Valente, A.]]
[[Category: Pilo-Veloso D]]
[[Category: Verly, R M.]]
[[Category: Resende JM]]
[[Category: Amphibian defense peptide]]
[[Category: Valente A]]
[[Category: Amphipathic alpha-helix]]
[[Category: Verly RM]]
[[Category: Antibiotic]]
[[Category: Antimicrobial]]
[[Category: Antimicrobial protein]]
[[Category: C-terminal carboxyamidation]]
[[Category: Membrane protein structure determination]]
[[Category: Secreted]]
Retrieved from "https://proteopedia.org/2k9b"