1wsj: Difference between revisions

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[[Image:1wsj.gif|left|200px]]<br /><applet load="1wsj" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1wsj, resolution 2.00&Aring;" />
'''Crystal structure of E.coli RNase HI active site mutant (K87A/H124A)'''<br />


==About this Structure==
==Crystal structure of E.coli RNase HI active site mutant (K87A/H124A)==
1WSJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Active as [http://en.wikipedia.org/wiki/Ribonuclease_H Ribonuclease H], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.4 3.1.26.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WSJ OCA].  
<StructureSection load='1wsj' size='340' side='right'caption='[[1wsj]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1wsj]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WSJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WSJ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wsj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wsj OCA], [https://pdbe.org/1wsj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wsj RCSB], [https://www.ebi.ac.uk/pdbsum/1wsj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wsj ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RNH_ECOLI RNH_ECOLI] Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. RNase H participates in DNA replication; it helps to specify the origin of genomic replication by suppressing initiation at origins other than the oriC locus; along with the 5'-3' exonuclease of pol1, it removes RNA primers from the Okazaki fragments of lagging strand synthesis; and it defines the origin of replication for ColE1-type plasmids by specific cleavage of an RNA preprimer.[HAMAP-Rule:MF_00042]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ws/1wsj_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wsj ConSurf].
<div style="clear:both"></div>
 
==See Also==
*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Ribonuclease H]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Kanaya S]]
[[Category: Kanaya, S.]]
[[Category: Matsumura H]]
[[Category: Matsumura, H.]]
[[Category: Takano K]]
[[Category: Takano, K.]]
[[Category: Tsunaka Y]]
[[Category: Tsunaka, Y.]]
[[Category: Yamagata Y]]
[[Category: Yamagata, Y.]]
[[Category: active-site mutant]]
[[Category: rnase h]]
[[Category: x-ray crystallography]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:47:40 2008''
Retrieved from "https://proteopedia.org/1wsj"