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[[Image:2hlv.png|left|200px]]


{{STRUCTURE_2hlv| PDB=2hlv | SCENE= }}
==Bovine Odorant Binding Protein deswapped triple mutant==
<StructureSection load='2hlv' size='340' side='right'caption='[[2hlv]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2hlv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HLV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HLV FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=LIK:3,6-BIS(METHYLENE)DECANOIC+ACID'>LIK</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hlv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hlv OCA], [https://pdbe.org/2hlv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hlv RCSB], [https://www.ebi.ac.uk/pdbsum/2hlv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hlv ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/OBP_BOVIN OBP_BOVIN] This protein binds a wide variety of chemical odorants.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hl/2hlv_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hlv ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The X-ray structure of bovine Odorant Binding Protein (bOBP) revealed its association as a domain swapped dimer. bOBP, devoid of any cysteines, contrasts with other mammalian OBPs, which are monomeric and possess at least one disulfide bridge. We have produced a mutant of bOBP in which a glycine residue was inserted after position 121. This mutation yielded a monomeric bOBP-121Gly+ in which domain swapping has been reverted. Here, we have subsequently introduced two mutations, Trp64Cys and His155Cys, in view to stabilize the putative monomer with a disulfide bridge. We have determined the crystal structure of this triple mutant at 1.65 A resolution. The mutant protein is monomeric, stabilized by a disulfide bridge between Trp64Cys and His155Cys, with a backbone superimposable to that of native bOBP, with the exception of the hinge and of the 10 residues at the C-terminus. bOBP triple mutant binds 1-amino-anthracene, 1-octen-3-ol (bOBP co-purified ligand) and other ligands with microM Kd values comparable to those of the swapped dimer.


===Bovine Odorant Binding Protein deswapped triple mutant===
Deswapping bovine odorant binding protein.,Ramoni R, Spinelli S, Grolli S, Conti V, Merli E, Cambillau C, Tegoni M Biochim Biophys Acta. 2008 Apr;1784(4):651-7. Epub 2008 Jan 29. PMID:18269920<ref>PMID:18269920</ref>


{{ABSTRACT_PUBMED_18269920}}
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
==About this Structure==
<div class="pdbe-citations 2hlv" style="background-color:#fffaf0;"></div>
[[2hlv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HLV OCA].


==See Also==
==See Also==
*[[Odorant binding protein|Odorant binding protein]]
*[[Odorant binding protein 3D structures|Odorant binding protein 3D structures]]
 
== References ==
==Reference==
<references/>
<ref group="xtra">PMID:018269920</ref><references group="xtra"/>
__TOC__
</StructureSection>
[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: Cambillau, C.]]
[[Category: Large Structures]]
[[Category: Spinelli, S.]]
[[Category: Cambillau C]]
[[Category: Tegoni, M.]]
[[Category: Spinelli S]]
[[Category: Domain swapping]]
[[Category: Tegoni M]]
[[Category: Odorant binding protein]]
[[Category: Transport protein]]
Retrieved from "https://proteopedia.org/2hlv"