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[[Image:2kyg.png|left|200px]]


{{STRUCTURE_2kyg| PDB=2kyg |  SCENE= }}
==Structure of the AML1-ETO Nervy Domain - PKA(RIIa) complex and its contribution to AML1-ETO activity==
<StructureSection load='2kyg' size='340' side='right'caption='[[2kyg]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2kyg]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KYG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KYG FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kyg OCA], [https://pdbe.org/2kyg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kyg RCSB], [https://www.ebi.ac.uk/pdbsum/2kyg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kyg ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/KAP2_HUMAN KAP2_HUMAN] Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
AML1-ETO is the chimeric protein product of t(8;21) in acute myeloid leukemia. The ETO portion of the fusion protein includes the nervy homology region (NHR) 3 domain, which shares homology with A-kinase anchoring proteins and interacts with the regulatory subunit of type II cAMP-dependent protein kinase A (PKA(RIIalpha)). We determined the solution structure of a complex between the AML1-ETO NHR3 domain and PKA(RIIalpha). Based on this structure, a key residue in AML1-ETO for PKA(RIIalpha) association was mutated. This mutation did not disrupt AML1-ETO's ability to enhance the clonogenic capacity of primary mouse bone marrow cells or its ability to repress proliferation or granulocyte differentiation. Introduction of the mutation into AML1-ETO had minimal impact on in vivo leukemogenesis. Therefore, the NHR3-PKA(RIIalpha) protein interaction does not appear to significantly contribute to AML1-ETO's ability to induce leukemia.


===Structure of the AML1-ETO Nervy Domain - PKA(RIIa) complex and its contribution to AML1-ETO activity===
Structure of the AML1-ETO NHR3-PKA(RIIalpha) complex and its contribution to AML1-ETO activity.,Corpora T, Roudaia L, Oo ZM, Chen W, Manuylova E, Cai X, Chen MJ, Cierpicki T, Speck NA, Bushweller JH J Mol Biol. 2010 Sep 24;402(3):560-77. Epub 2010 Aug 11. PMID:20708017<ref>PMID:20708017</ref>


{{ABSTRACT_PUBMED_20708017}}
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
==About this Structure==
<div class="pdbe-citations 2kyg" style="background-color:#fffaf0;"></div>
[[2kyg]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KYG OCA].


==See Also==
==See Also==
*[[CAMP-dependent protein kinase|CAMP-dependent protein kinase]]
*[[CAMP-dependent protein kinase 3D structures|CAMP-dependent protein kinase 3D structures]]
 
== References ==
==Reference==
<references/>
<ref group="xtra">PMID:020708017</ref><references group="xtra"/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Bushweller, J.]]
[[Category: Large Structures]]
[[Category: Cierpecki, T.]]
[[Category: Bushweller J]]
[[Category: Corpora, T A.]]
[[Category: Cierpecki T]]
[[Category: Homodimer bound to monomer]]
[[Category: Corpora TA]]
[[Category: Protein binding]]
[[Category: Protein/protein]]
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