2ns3: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
m Protected "2ns3" [edit=sysop:move=sysop]
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
No edit summary
 
(7 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:2ns3.png|left|200px]]


{{STRUCTURE_2ns3| PDB=2ns3 |  SCENE= }}
==Solution structure of ribbon BuIA==
<StructureSection load='2ns3' size='340' side='right'caption='[[2ns3]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2ns3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Conus_bullatus Conus bullatus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NS3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NS3 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ns3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ns3 OCA], [https://pdbe.org/2ns3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ns3 RCSB], [https://www.ebi.ac.uk/pdbsum/2ns3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ns3 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CA1A_CONBU CA1A_CONBU] Alpha-conotoxins bind to the nicotinic acetylcholine receptors (nAChR) and inhibit them. This peptide potently blocks numerous mammalian nAChR subtypes (alpha-6 or -3/beta-2 or -3 > alpha-6 or-3/beta-4 > alpha-3/beta-2 > alpha-3/beta-4 > alpha-4/beta-4 = alpha-2/beta-4 > alpha-7 > alpha-2/beta-2 >> alpha-4/beta-2). Recovery from toxin block is markedly slower for beta-4 versus beta-2 subunit-containing nAChRs. Thus, it represents a novel probe for distinguishing between beta-2 and beta-4-containing nAChRs.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
BACKGROUND: Alpha-conotoxins have exciting therapeutic potential based on their high selectivity and affinity for nicotinic acetylcholine receptors. The spacing between the cysteine residues in alpha-conotoxins is variable, leading to the classification of sub-families. BuIA is the only alpha-conotoxin containing a 4/4 cysteine spacing and thus it is of significant interest to examine the structure of this conotoxin. RESULTS: In the current study we show the native globular disulfide connectivity of BuIA displays multiple conformations in solution whereas the non-native ribbon isomer has a single well-defined conformation. Despite having multiple conformations in solution the globular form of BuIA displays activity at the nicotinic acetylcholine receptor, contrasting with the lack of activity of the structurally well-defined ribbon isomer. CONCLUSION: These findings are opposite to the general trends observed for alpha-conotoxins where the native isomers have well-defined structures and the ribbon isomers are generally disordered. This study thus highlights the influence of the disulfide connectivity of BuIA on the dynamics of the three-dimensional structure.


===Solution structure of ribbon BuIA===
Structure of alpha-conotoxin BuIA: influences of disulfide connectivity on structural dynamics.,Jin AH, Brandstaetter H, Nevin ST, Tan CC, Clark RJ, Adams DJ, Alewood PF, Craik DJ, Daly NL BMC Struct Biol. 2007 Apr 20;7:28. PMID:17445276<ref>PMID:17445276</ref>


{{ABSTRACT_PUBMED_17445276}}
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
==About this Structure==
<div class="pdbe-citations 2ns3" style="background-color:#fffaf0;"></div>
[[2ns3]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NS3 OCA].
== References ==
[[Category: Adams, D J.]]
<references/>
[[Category: Alewood, P F.]]
__TOC__
[[Category: Brandstaetter, H.]]
</StructureSection>
[[Category: Clark, R J.]]
[[Category: Conus bullatus]]
[[Category: Craik, D J.]]
[[Category: Large Structures]]
[[Category: Daly, N L.]]
[[Category: Adams DJ]]
[[Category: Jin, A H.]]
[[Category: Alewood PF]]
[[Category: Nevin, S T.]]
[[Category: Brandstaetter H]]
[[Category: Tan, C C.]]
[[Category: Clark RJ]]
[[Category: Ribbon disulfide connectivity]]
[[Category: Craik DJ]]
[[Category: Toxin]]
[[Category: Daly NL]]
[[Category: Jin AH]]
[[Category: Nevin ST]]
[[Category: Tan CC]]
Retrieved from "https://proteopedia.org/2ns3"