3ddz: Difference between revisions

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[[Image:3ddz.png|left|200px]]


{{STRUCTURE_3ddz| PDB=3ddz | SCENE= }}
==Proteinase K by LB nanotemplate method after the first step of high X-Ray dose on ESRF ID23-1 beamline==
<StructureSection load='3ddz' size='340' side='right'caption='[[3ddz]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3ddz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Parengyodontium_album Parengyodontium album]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DDZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3DDZ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ddz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ddz OCA], [https://pdbe.org/3ddz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ddz RCSB], [https://www.ebi.ac.uk/pdbsum/3ddz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ddz ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PRTK_PARAQ PRTK_PARAQ] Hydrolyzes keratin at aromatic and hydrophobic residues.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dd/3ddz_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ddz ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
A detailed analysis of structural and intensity changes induced by X-ray radiation is presented for two types of proteinase K crystals: crystal grown by classical hanging drop method and those grown by Langmuir-Blodgett (LB) nanotemplate. The comparison of various parameters (e.g. intensity per sigma ratio, unit-cell volume, number of unique reflections, B-factors) and electron density maps as a function of radiation dose, demonstrates that crystals, grown by the LB nanotemplate method, appear to be more resistant against radiation damage than crystals grown by the classical hanging drop method.


===Proteinase K by LB nanotemplate method after the first step of high X-Ray dose on ESRF ID23-1 beamline===
Radiation stability of proteinase K crystals grown by LB nanotemplate method.,Pechkova E, Tripathi S, Ravelli RB, McSweeney S, Nicolini C J Struct Biol. 2009 Dec;168(3):409-18. Epub 2009 Aug 15. PMID:19686853<ref>PMID:19686853</ref>


{{ABSTRACT_PUBMED_19686853}}
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3ddz" style="background-color:#fffaf0;"></div>


==About this Structure==
==See Also==
[[3ddz]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Engyodontium_album Engyodontium album]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DDZ OCA].
*[[Proteinase 3D structures|Proteinase 3D structures]]
 
== References ==
==Reference==
<references/>
<ref group="xtra">PMID:019686853</ref><references group="xtra"/>
__TOC__
[[Category: Engyodontium album]]
</StructureSection>
[[Category: Peptidase K]]
[[Category: Large Structures]]
[[Category: Nicolini, C.]]
[[Category: Parengyodontium album]]
[[Category: Pechkova, E.]]
[[Category: Nicolini C]]
[[Category: Tripathi, S K.]]
[[Category: Pechkova E]]
[[Category: Alpha and beta protein]]
[[Category: Tripathi SK]]
[[Category: Hydrolase]]
[[Category: Metal-binding]]
[[Category: Protease]]
[[Category: Serine protease]]
[[Category: Subtilase]]
[[Category: Zymogen]]

Latest revision as of 09:47, 6 November 2024

Proteinase K by LB nanotemplate method after the first step of high X-Ray dose on ESRF ID23-1 beamline

3ddz, resolution 1.70Å

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