3b9p: Difference between revisions

Latest revision as of 09:28, 21 February 2024

Spastin

Structural highlights

3b9p is a 1 chain structure with sequence from Drosophila melanogaster. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.7Å
Ligands:	CL
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SPAST_DROME ATP-dependent microtubule severing protein. Stimulates microtubule minus-end depolymerization and poleward microtubule flux in the mitotic spindle. Regulates microtubule stability in the neuromuscular junction synapse.^[1] ^[2] ^[3] ^[4] ^[5] ^[6] ^[7]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

↑ Trotta N, Orso G, Rossetto MG, Daga A, Broadie K. The hereditary spastic paraplegia gene, spastin, regulates microtubule stability to modulate synaptic structure and function. Curr Biol. 2004 Jul 13;14(13):1135-47. PMID:15242610 doi:https://dx.doi.org/10.1016/j.cub.2004.06.058
↑ Sherwood NT, Sun Q, Xue M, Zhang B, Zinn K. Drosophila spastin regulates synaptic microtubule networks and is required for normal motor function. PLoS Biol. 2004 Dec;2(12):e429. Epub 2004 Nov 30. PMID:15562320 doi:https://dx.doi.org/10.1371/journal.pbio.0020429
↑ Roll-Mecak A, Vale RD. The Drosophila homologue of the hereditary spastic paraplegia protein, spastin, severs and disassembles microtubules. Curr Biol. 2005 Apr 12;15(7):650-5. PMID:15823537 doi:https://dx.doi.org/S0960-9822(05)00170-3
↑ Orso G, Martinuzzi A, Rossetto MG, Sartori E, Feany M, Daga A. Disease-related phenotypes in a Drosophila model of hereditary spastic paraplegia are ameliorated by treatment with vinblastine. J Clin Invest. 2005 Nov;115(11):3026-34. PMID:16276413 doi:https://dx.doi.org/10.1172/JCI24694
↑ Zhang D, Rogers GC, Buster DW, Sharp DJ. Three microtubule severing enzymes contribute to the "Pacman-flux" machinery that moves chromosomes. J Cell Biol. 2007 Apr 23;177(2):231-42. PMID:17452528 doi:https://dx.doi.org/jcb.200612011
↑ Lee M, Paik SK, Lee MJ, Kim YJ, Kim S, Nahm M, Oh SJ, Kim HM, Yim J, Lee CJ, Bae YC, Lee S. Drosophila Atlastin regulates the stability of muscle microtubules and is required for synapse development. Dev Biol. 2009 Jun 15;330(2):250-62. doi: 10.1016/j.ydbio.2009.03.019. Epub 2009 , Mar 31. PMID:19341724 doi:https://dx.doi.org/10.1016/j.ydbio.2009.03.019
↑ Roll-Mecak A, Vale RD. Structural basis of microtubule severing by the hereditary spastic paraplegia protein spastin. Nature. 2008 Jan 17;451(7176):363-7. PMID:18202664 doi:10.1038/nature06482

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Trotta N, Orso G, Rossetto MG, Daga A, Broadie K. The hereditary spastic paraplegia gene, spastin, regulates microtubule stability to modulate synaptic structure and function. Curr Biol. 2004 Jul 13;14(13):1135-47. PMID:15242610 doi:https://dx.doi.org/10.1016/j.cub.2004.06.058

[2] Sherwood NT, Sun Q, Xue M, Zhang B, Zinn K. Drosophila spastin regulates synaptic microtubule networks and is required for normal motor function. PLoS Biol. 2004 Dec;2(12):e429. Epub 2004 Nov 30. PMID:15562320 doi:https://dx.doi.org/10.1371/journal.pbio.0020429

[3] Roll-Mecak A, Vale RD. The Drosophila homologue of the hereditary spastic paraplegia protein, spastin, severs and disassembles microtubules. Curr Biol. 2005 Apr 12;15(7):650-5. PMID:15823537 doi:https://dx.doi.org/S0960-9822(05)00170-3

[4] Orso G, Martinuzzi A, Rossetto MG, Sartori E, Feany M, Daga A. Disease-related phenotypes in a Drosophila model of hereditary spastic paraplegia are ameliorated by treatment with vinblastine. J Clin Invest. 2005 Nov;115(11):3026-34. PMID:16276413 doi:https://dx.doi.org/10.1172/JCI24694

[5] Zhang D, Rogers GC, Buster DW, Sharp DJ. Three microtubule severing enzymes contribute to the "Pacman-flux" machinery that moves chromosomes. J Cell Biol. 2007 Apr 23;177(2):231-42. PMID:17452528 doi:https://dx.doi.org/jcb.200612011

[6] Lee M, Paik SK, Lee MJ, Kim YJ, Kim S, Nahm M, Oh SJ, Kim HM, Yim J, Lee CJ, Bae YC, Lee S. Drosophila Atlastin regulates the stability of muscle microtubules and is required for synapse development. Dev Biol. 2009 Jun 15;330(2):250-62. doi: 10.1016/j.ydbio.2009.03.019. Epub 2009 , Mar 31. PMID:19341724 doi:https://dx.doi.org/10.1016/j.ydbio.2009.03.019

[7] Roll-Mecak A, Vale RD. Structural basis of microtubule severing by the hereditary spastic paraplegia protein spastin. Nature. 2008 Jan 17;451(7176):363-7. PMID:18202664 doi:10.1038/nature06482

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@@ Line 1: / Line 1: @@
-[[Image:3b9p.png|left|200px]]
-{{STRUCTURE_3b9p|  PDB=3b9p  |  SCENE=  }}
+==Spastin==
+<StructureSection load='3b9p' size='340' side='right'caption='[[3b9p]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
-===Spastin===
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3b9p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B9P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3B9P FirstGlance]. <br>
-{{ABSTRACT_PUBMED_18202664}}
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-==About this Structure==
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3b9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b9p OCA], [https://pdbe.org/3b9p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3b9p RCSB], [https://www.ebi.ac.uk/pdbsum/3b9p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3b9p ProSAT]</span></td></tr>
-[[3b9p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B9P OCA].
+</table>
+== Function ==
-==Reference==
+[https://www.uniprot.org/uniprot/SPAST_DROME SPAST_DROME] ATP-dependent microtubule severing protein. Stimulates microtubule minus-end depolymerization and poleward microtubule flux in the mitotic spindle. Regulates microtubule stability in the neuromuscular junction synapse.<ref>PMID:15242610</ref> <ref>PMID:15562320</ref> <ref>PMID:15823537</ref> <ref>PMID:16276413</ref> <ref>PMID:17452528</ref> <ref>PMID:19341724</ref> <ref>PMID:18202664</ref>
-<ref group="xtra">PMID:018202664</ref><references group="xtra"/>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b9/3b9p_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3b9p ConSurf].
+<div style="clear:both"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Drosophila melanogaster]]
-[[Category: Roll-Mecak, A.]]
+[[Category: Large Structures]]
-[[Category: Vale, R D.]]
+[[Category: Roll-Mecak A]]
-[[Category: Aaa atpase]]
+[[Category: Vale RD]]
-[[Category: Atp-binding]]
-[[Category: Hydrolase]]
-[[Category: Nucleotide-binding]]

3b9p: Difference between revisions

Latest revision as of 09:28, 21 February 2024

Spastin

Structural highlights

Function

Evolutionary Conservation

References

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