3c7t: Difference between revisions

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[[Image:3c7t.png|left|200px]]


{{STRUCTURE_3c7t| PDB=3c7t | SCENE= }}
==Crystal structure of the ecdysone phosphate phosphatase, EPPase, from Bombix mori in complex with tungstate==
<StructureSection load='3c7t' size='340' side='right'caption='[[3c7t]], [[Resolution|resolution]] 1.76&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3c7t]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bombyx_mori Bombyx mori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C7T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3C7T FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.76&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=WO4:TUNGSTATE(VI)ION'>WO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3c7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c7t OCA], [https://pdbe.org/3c7t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3c7t RCSB], [https://www.ebi.ac.uk/pdbsum/3c7t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3c7t ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/EPP_BOMMO EPP_BOMMO] Steroid phosphatase which catalyzes the conversion of inactive phosphorylated ecdysteroids into their active forms (PubMed:12721294, PubMed:15738639, PubMed:18937503). Shows high activity towards ecdysone 22-phosphate (E22P) (PubMed:12721294). Has lower activity towards other ecdysteriod phosphates including 20-hydroxyecdysone 22-phosphate (20E22P) and 2-deoxyecdysone 22-phosphate (2dE22P) (PubMed:12721294). Also has protein tyrosine phosphatase activity (PubMed:18937503).<ref>PMID:12721294</ref> <ref>PMID:15738639</ref> <ref>PMID:18937503</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c7/3c7t_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3c7t ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Here, we present the crystal structure of the ecdysone phosphate phosphatase (EPPase) phosphoglycerate mutase (PGM) homology domain, the first structure of a steroid phosphate phosphatase. The structure reveals an alpha/beta-fold common to members of the two histidine (2H)-phosphatase superfamily with strong homology to the Suppressor of T-cell receptor signaling-1 (Sts-1 PGM) protein. The putative EPPase PGM active site contains signature residues shared by 2H-phosphatase enzymes, including a conserved histidine (His80) that acts as a nucleophile during catalysis. The physiological substrate ecdysone 22-phosphate was modeled in a hydrophobic cavity close to the phosphate-binding site. EPPase PGM shows limited substrate specificity with an ability to hydrolyze steroid phosphates, the phospho-tyrosine (pTyr) substrate analogue para-nitrophenylphosphate ( pNPP) and pTyr-containing peptides and proteins. Altogether, our data demonstrate a new protein tyrosine phosphatase (PTP) activity for EPPase. They suggest that EPPase and its closest homologues can be grouped into a distinct subfamily in the large 2H-phosphatase superfamily of proteins.


===Crystal structure of the ecdysone phosphate phosphatase, EPPase, from Bombix mori in complex with tungstate===
Structural and functional characterization of the c-terminal domain of the ecdysteroid phosphate phosphatase from bombyx mori reveals a new enzymatic activity.,Chen Y, Jakoncic J, Wang J, Zheng X, Carpino N, Nassar N Biochemistry. 2008 Nov 18;47(46):12135-45. Epub 2008 Oct 21. PMID:18937503<ref>PMID:18937503</ref>


{{ABSTRACT_PUBMED_18937503}}
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
==About this Structure==
<div class="pdbe-citations 3c7t" style="background-color:#fffaf0;"></div>
[[3c7t]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bombyx_mori Bombyx mori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C7T OCA].
== References ==
 
<references/>
==Reference==
__TOC__
<ref group="xtra">PMID:018937503</ref><references group="xtra"/>
</StructureSection>
[[Category: Bombyx mori]]
[[Category: Bombyx mori]]
[[Category: Carpino, N.]]
[[Category: Large Structures]]
[[Category: Chen, Y.]]
[[Category: Carpino N]]
[[Category: Nassar, N.]]
[[Category: Chen Y]]
[[Category: 2h-phosphatase]]
[[Category: Nassar N]]
[[Category: Ecdysone]]
[[Category: Hydrolase]]
[[Category: Pgm]]
[[Category: Phosphatase]]
Retrieved from "https://proteopedia.org/3c7t"