2r19: Difference between revisions

Latest revision as of 09:19, 21 February 2024

Crystal structure of the periplasmic lipopolysaccharide transport protein LptA (YhbN), orthorhombic form

Structural highlights

2r19 is a 2 chain structure with sequence from Escherichia coli K-12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.16Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

LPTA_ECOLI Involved in the assembly of lipopolysaccharide (LPS). Required for the translocation of LPS from the inner membrane to the outer membrane. May form a bridge between the inner membrane and the outer membrane, via interactions with LptC and LptD, thereby facilitating LPS transfer across the periplasm.[HAMAP-Rule:MF_01914]^[1] ^[2] ^[3] ^[4] ^[5]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

↑ Sperandeo P, Pozzi C, Deho G, Polissi A. Non-essential KDO biosynthesis and new essential cell envelope biogenesis genes in the Escherichia coli yrbG-yhbG locus. Res Microbiol. 2006 Jul-Aug;157(6):547-58. Epub 2006 Feb 9. PMID:16765569 doi:10.1016/j.resmic.2005.11.014
↑ Sperandeo P, Cescutti R, Villa R, Di Benedetto C, Candia D, Deho G, Polissi A. Characterization of lptA and lptB, two essential genes implicated in lipopolysaccharide transport to the outer membrane of Escherichia coli. J Bacteriol. 2007 Jan;189(1):244-53. Epub 2006 Oct 20. PMID:17056748 doi:https://dx.doi.org/10.1128/JB.01126-06
↑ Sperandeo P, Lau FK, Carpentieri A, De Castro C, Molinaro A, Deho G, Silhavy TJ, Polissi A. Functional analysis of the protein machinery required for transport of lipopolysaccharide to the outer membrane of Escherichia coli. J Bacteriol. 2008 Jul;190(13):4460-9. Epub 2008 Apr 18. PMID:18424520 doi:JB.00270-08
↑ Tran AX, Trent MS, Whitfield C. The LptA protein of Escherichia coli is a periplasmic lipid A-binding protein involved in the lipopolysaccharide export pathway. J Biol Chem. 2008 Jul 18;283(29):20342-9. doi: 10.1074/jbc.M802503200. Epub 2008 , May 14. PMID:18480051 doi:https://dx.doi.org/10.1074/jbc.M802503200
↑ Sperandeo P, Villa R, Martorana AM, Samalikova M, Grandori R, Deho G, Polissi A. New insights into the Lpt machinery for lipopolysaccharide transport to the cell surface: LptA-LptC interaction and LptA stability as sensors of a properly assembled transenvelope complex. J Bacteriol. 2011 Mar;193(5):1042-53. doi: 10.1128/JB.01037-10. Epub 2010 Dec 17. PMID:21169485 doi:https://dx.doi.org/10.1128/JB.01037-10

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Sperandeo P, Pozzi C, Deho G, Polissi A. Non-essential KDO biosynthesis and new essential cell envelope biogenesis genes in the Escherichia coli yrbG-yhbG locus. Res Microbiol. 2006 Jul-Aug;157(6):547-58. Epub 2006 Feb 9. PMID:16765569 doi:10.1016/j.resmic.2005.11.014

[2] Sperandeo P, Cescutti R, Villa R, Di Benedetto C, Candia D, Deho G, Polissi A. Characterization of lptA and lptB, two essential genes implicated in lipopolysaccharide transport to the outer membrane of Escherichia coli. J Bacteriol. 2007 Jan;189(1):244-53. Epub 2006 Oct 20. PMID:17056748 doi:https://dx.doi.org/10.1128/JB.01126-06

[3] Sperandeo P, Lau FK, Carpentieri A, De Castro C, Molinaro A, Deho G, Silhavy TJ, Polissi A. Functional analysis of the protein machinery required for transport of lipopolysaccharide to the outer membrane of Escherichia coli. J Bacteriol. 2008 Jul;190(13):4460-9. Epub 2008 Apr 18. PMID:18424520 doi:JB.00270-08

[4] Tran AX, Trent MS, Whitfield C. The LptA protein of Escherichia coli is a periplasmic lipid A-binding protein involved in the lipopolysaccharide export pathway. J Biol Chem. 2008 Jul 18;283(29):20342-9. doi: 10.1074/jbc.M802503200. Epub 2008 , May 14. PMID:18480051 doi:https://dx.doi.org/10.1074/jbc.M802503200

[5] Sperandeo P, Villa R, Martorana AM, Samalikova M, Grandori R, Deho G, Polissi A. New insights into the Lpt machinery for lipopolysaccharide transport to the cell surface: LptA-LptC interaction and LptA stability as sensors of a properly assembled transenvelope complex. J Bacteriol. 2011 Mar;193(5):1042-53. doi: 10.1128/JB.01037-10. Epub 2010 Dec 17. PMID:21169485 doi:https://dx.doi.org/10.1128/JB.01037-10

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[2]

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[5]

@@ Line 1: / Line 1: @@
-[[Image:2r19.png|left|200px]]
-{{STRUCTURE_2r19|  PDB=2r19  |  SCENE=  }}
+==Crystal structure of the periplasmic lipopolysaccharide transport protein LptA (YhbN), orthorhombic form==
+<StructureSection load='2r19' size='340' side='right'caption='[[2r19]], [[Resolution|resolution]] 2.16&Aring;' scene=''>
-===Crystal structure of the periplasmic lipopolysaccharide transport protein LptA (YhbN), orthorhombic form===
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2r19]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R19 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2R19 FirstGlance]. <br>
-{{ABSTRACT_PUBMED_18534617}}
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.16&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2r19 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r19 OCA], [https://pdbe.org/2r19 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2r19 RCSB], [https://www.ebi.ac.uk/pdbsum/2r19 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2r19 ProSAT]</span></td></tr>
-==About this Structure==
+</table>
-[[2r19]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R19 OCA].
+== Function ==
+[https://www.uniprot.org/uniprot/LPTA_ECOLI LPTA_ECOLI] Involved in the assembly of lipopolysaccharide (LPS). Required for the translocation of LPS from the inner membrane to the outer membrane. May form a bridge between the inner membrane and the outer membrane, via interactions with LptC and LptD, thereby facilitating LPS transfer across the periplasm.[HAMAP-Rule:MF_01914]<ref>PMID:16765569</ref> <ref>PMID:17056748</ref> <ref>PMID:18424520</ref> <ref>PMID:18480051</ref> <ref>PMID:21169485</ref>
-==Reference==
+== Evolutionary Conservation ==
-<ref group="xtra">PMID:018534617</ref><references group="xtra"/>
+[[Image:Consurf_key_small.gif|200px|right]]
-[[Category: Escherichia coli k-12]]
+Check<jmol>
-[[Category: BSGI, Montreal-Kingston Bacterial Structural Genomics Initiative.]]
+  <jmolCheckbox>
-[[Category: Jia, Z.]]
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r1/2r19_consurf.spt"</scriptWhenChecked>
-[[Category: Polissi, A.]]
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-[[Category: Suits, M D.L.]]
+    <text>to colour the structure by Evolutionary Conservation</text>
-[[Category: Beta-jellyroll]]
+  </jmolCheckbox>
-[[Category: Beta-taco]]
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2r19 ConSurf].
-[[Category: Bsgi]]
+<div style="clear:both"></div>
-[[Category: Mainly beta]]
+== References ==
-[[Category: Montreal-kingston bacterial structural genomics initiative]]
+<references/>
-[[Category: Structural genomic]]
+__TOC__
-[[Category: Transport protein]]
+</StructureSection>
+[[Category: Escherichia coli K-12]]
+[[Category: Large Structures]]
+[[Category: Jia Z]]
+[[Category: Polissi A]]
+[[Category: Suits MDL]]

2r19: Difference between revisions

Latest revision as of 09:19, 21 February 2024

Crystal structure of the periplasmic lipopolysaccharide transport protein LptA (YhbN), orthorhombic form

Structural highlights

Function

Evolutionary Conservation

References

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