2w6k: Difference between revisions

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[[Image:2w6k.png|left|200px]]


{{STRUCTURE_2w6k| PDB=2w6k | SCENE= }}
==The crystal structure at 1.7 A resolution of CobE, a protein from the cobalamin (vitamin B12) biosynthetic pathway==
<StructureSection load='2w6k' size='340' side='right'caption='[[2w6k]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2w6k]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2bsn 2bsn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W6K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W6K FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2w6l|2w6l]]</div></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w6k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w6k OCA], [https://pdbe.org/2w6k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w6k RCSB], [https://www.ebi.ac.uk/pdbsum/2w6k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w6k ProSAT]</span></td></tr>
</table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w6/2w6k_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2w6k ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
CobE, a protein implicated in vitamin B12 biosynthesis, from Pseudomonas aeruginosa has been overexpressed in Escherichia coli, purified and crystallized using hanging-drop vapour diffusion. The crystals belong to the primitive orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 31.86, b = 41.07, c = 87.41 A. The diffraction extends to a resolution of 1.9 A. There is one molecule per asymmetric unit and the estimated solvent content is 35%. SeMet-labelled CobE has been prepared and crystallizes under the same conditions as the native protein with diffraction to 1.7 A. The anomalous measurements will be used for phasing.


===THE CRYSTAL STRUCTURE AT 1.7 A RESOLUTION OF COBE, A PROTEIN FROM THE COBALAMIN (VITAMIN B12) BIOSYNTHETIC PATHWAY===
Crystallization and preliminary structure analysis of CobE, an essential protein of cobalamin (vitamin B12) biosynthesis.,Vevodova J, Graham RM, Raux E, Warren MJ, Wilson KS Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Apr 1;61(Pt, 4):442-4. Epub 2005 Apr 1. PMID:16511064<ref>PMID:16511064</ref>


{{ABSTRACT_PUBMED_16511064}}
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
==About this Structure==
<div class="pdbe-citations 2w6k" style="background-color:#fffaf0;"></div>
[[2w6k]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2bsn 2bsn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W6K OCA].
== References ==
 
<references/>
==Reference==
__TOC__
<ref group="xtra">PMID:016511064</ref><references group="xtra"/>
</StructureSection>
[[Category: Pseudomonas aeruginosa]]
[[Category: Large Structures]]
[[Category: Deery, E.]]
[[Category: Deery, E]]
[[Category: Mcgoldrick, H.]]
[[Category: McGoldrick, H]]
[[Category: Murzin, A G.]]
[[Category: Murzin, A G]]
[[Category: Smith, D.]]
[[Category: Smith, D]]
[[Category: Vevodova, J.]]
[[Category: Vevodova, J]]
[[Category: Warren, M J.]]
[[Category: Warren, M J]]
[[Category: Wilson, K S.]]
[[Category: Wilson, K S]]
[[Category: Biosynthetic protein]]
[[Category: Biosynthetic protein]]
[[Category: Cobalamin]]
[[Category: Cobalamin]]

Latest revision as of 10:58, 6 April 2022

The crystal structure at 1.7 A resolution of CobE, a protein from the cobalamin (vitamin B12) biosynthetic pathway

2w6k, resolution 1.70Å

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