3dp5: Difference between revisions

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[[Image:3dp5.png|left|200px]]


{{STRUCTURE_3dp5| PDB=3dp5 | SCENE= }}
==Crystal structure of Geobacter sulfurreducens OmcF with N-terminal Strep-tag II==
<StructureSection load='3dp5' size='340' side='right'caption='[[3dp5]], [[Resolution|resolution]] 1.86&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3dp5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacter_sulfurreducens Geobacter sulfurreducens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DP5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3DP5 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.86&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3dp5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dp5 OCA], [https://pdbe.org/3dp5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3dp5 RCSB], [https://www.ebi.ac.uk/pdbsum/3dp5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3dp5 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q74AE4_GEOSL Q74AE4_GEOSL]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dp/3dp5_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3dp5 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The putative outer membrane c-type cytochrome OmcF from Geobacter sulfurreducens contains a single haem group and shows homology to soluble cytochromes c(6), a class of electron-transfer proteins that are typically found in cyanobacterial photosynthetic electron-transfer chains. OmcF was overexpressed heterologously in Escherichia coli as an N-terminal Strep-tag II fusion protein and isolated using streptactin-affinity chromatography followed by size-exclusion chromatography. The structure was solved by Fe SAD using data collected to a resolution of 1.86 A on a rotating copper-anode X-ray generator. In the crystal, packing interactions in one dimension were exclusively mediated through the Strep-tag II sequence. The tag and linker regions were in contact with three further monomers of OmcF, leading to a well defined electron-density map for this engineered and secondary-structure-free region of the molecule.


===Crystal structure of Geobacter sulfurreducens OmcF with N-terminal Strep-tag II===
Crystal packing of the c(6)-type cytochrome OmcF from Geobacter sulfurreducens is mediated by an N-terminal Strep-tag II.,Lukat P, Hoffmann M, Einsle O Acta Crystallogr D Biol Crystallogr. 2008 Sep;64(Pt 9):919-26. Epub 2008, Aug 13. PMID:18703839<ref>PMID:18703839</ref>


{{ABSTRACT_PUBMED_18703839}}
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
==About this Structure==
<div class="pdbe-citations 3dp5" style="background-color:#fffaf0;"></div>
[[3dp5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Geobacter_sulfurreducens Geobacter sulfurreducens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DP5 OCA].
== References ==
 
<references/>
==Reference==
__TOC__
<ref group="xtra">PMID:018703839</ref><references group="xtra"/>
</StructureSection>
[[Category: Geobacter sulfurreducens]]
[[Category: Geobacter sulfurreducens]]
[[Category: Einsle, O.]]
[[Category: Large Structures]]
[[Category: Hoffmann, M.]]
[[Category: Einsle O]]
[[Category: Lukat, P.]]
[[Category: Hoffmann M]]
[[Category: C-type cytochrome]]
[[Category: Lukat P]]
[[Category: Dissimilatory metal reduction]]
[[Category: Electron transport]]
[[Category: Fe sad phasing]]
[[Category: Geobacter sulfurreducen]]

Latest revision as of 09:00, 30 October 2024

Crystal structure of Geobacter sulfurreducens OmcF with N-terminal Strep-tag II

3dp5, resolution 1.86Å

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