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[[Image:1xs5.gif|left|200px]]<br /><applet load="1xs5" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1xs5, resolution 1.85&Aring;" />
'''The Crystal Structure of Lipoprotein Tp32 from Treponema pallidum'''<br />


==Overview==
==The Crystal Structure of Lipoprotein Tp32 from Treponema pallidum==
A structure-to-function approach was undertaken to gain insights into the potential function of the 32-kDa membrane lipoprotein (Tp32) of Treponema pallidum, the syphilis bacterium. The crystal structure of rTp32 (determined at a resolution of 1.85 A) shows that the organization of rTp32 is similar to other periplasmic ligand-binding proteins (PLBPs), in that it consists of two alpha/beta domains, linked by two crossovers, with a binding pocket between them. In the pocket, a molecule of L-methionine was detected in the electron density map. Residues from both domains interact with the ligand. One of the crossover regions is comprised of a 3(10)-helix, a feature not typical in other ligand-binding proteins. Sequence comparison shows strong similarity to other hypothetical methionine-binding proteins. Together, the data support the notion that rTp32 is a component of a periplasmic methionine uptake transporter system in T. pallidum.
<StructureSection load='1xs5' size='340' side='right'caption='[[1xs5]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1xs5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Treponema_pallidum Treponema pallidum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XS5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XS5 FirstGlance]. <br>
1XS5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Treponema_pallidum Treponema pallidum] with <scene name='pdbligand=MET:'>MET</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XS5 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MET:METHIONINE'>MET</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xs5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xs5 OCA], [https://pdbe.org/1xs5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xs5 RCSB], [https://www.ebi.ac.uk/pdbsum/1xs5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xs5 ProSAT]</span></td></tr>
Structural evidence that the 32-kilodalton lipoprotein (Tp32) of Treponema pallidum is an L-methionine-binding protein., Deka RK, Neil L, Hagman KE, Machius M, Tomchick DR, Brautigam CA, Norgard MV, J Biol Chem. 2004 Dec 31;279(53):55644-50. Epub 2004 Oct 15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15489229 15489229]
</table>
[[Category: Single protein]]
== Function ==
[https://www.uniprot.org/uniprot/TPN32_TREPA TPN32_TREPA]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xs/1xs5_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xs5 ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Treponema pallidum]]
[[Category: Treponema pallidum]]
[[Category: Brautigam, C A.]]
[[Category: Brautigam CA]]
[[Category: Deka, R K.]]
[[Category: Deka RK]]
[[Category: Hagman, K E.]]
[[Category: Hagman KE]]
[[Category: Machius, M.]]
[[Category: Machius M]]
[[Category: Neil, L.]]
[[Category: Neil L]]
[[Category: Norgard, M V.]]
[[Category: Norgard MV]]
[[Category: Tomchick, D R.]]
[[Category: Tomchick DR]]
[[Category: MET]]
[[Category: lipoprotein]]
[[Category: methionine]]
[[Category: periplasmic binding protein]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:58:08 2008''
Retrieved from "https://proteopedia.org/1xs5"