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[[Image:3bsy.png|left|200px]]


{{STRUCTURE_3bsy| PDB=3bsy | SCENE= }}
==PglD from Campylobacter jejuni, NCTC 11168, in complex with acetyl coenzyme A==
<StructureSection load='3bsy' size='340' side='right'caption='[[3bsy]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3bsy]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Campylobacter_jejuni Campylobacter jejuni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BSY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BSY FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bsy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bsy OCA], [https://pdbe.org/3bsy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bsy RCSB], [https://www.ebi.ac.uk/pdbsum/3bsy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bsy ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PGLD_CAMJE PGLD_CAMJE] Acetyltransferase that modifies the UDP-4-amino-sugar to form UDP-N,N'-diacetylbacillosamine in the N-linked protein glycosylation pathway.<ref>PMID:17087520</ref> <ref>PMID:19448740</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bs/3bsy_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bsy ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The carbohydrate 2, 4-diacetamido-2, 4, 6-trideoxy-alpha-D-glucopyranose (BacAc(2)) is found in a variety of eubacterial pathogens. In Campylobacter jejuni, PglD acetylates the C4 amino group on UDP-2-acetamido-4-amino-2, 4, 6-trideoxy-alpha-D-glucopyranose (UDP-4-amino-sugar) to form UDP-BacAc(2). Sequence analysis predicts PglD to be a member of the left-handed beta helix family of enzymes. However, poor sequence homology between PglD and left-handed beta helix enzymes with existing structural data precludes unambiguous identification of the active site. The co-crystal structures of PglD in the presence of citrate, acetyl coenzyme A, or the UDP-4-amino-sugar were solved. The biological assembly is a trimer with one active site formed between two protomers. Residues lining the active site were identified, and results from functional assays on alanine mutants suggest His-125 is critical for catalysis, whereas His-15 and His-134 are involved in substrate binding. These results are discussed in the context of implications for proteins homologous to PglD in other pathogens.


===PglD from Campylobacter jejuni, NCTC 11168, in complex with acetyl coenzyme A===
Crystal structure and catalytic mechanism of PglD from Campylobacter jejuni.,Olivier NB, Imperiali B J Biol Chem. 2008 Oct 10;283(41):27937-46. Epub 2008 Jul 30. PMID:18667421<ref>PMID:18667421</ref>


{{ABSTRACT_PUBMED_18667421}}
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
==About this Structure==
<div class="pdbe-citations 3bsy" style="background-color:#fffaf0;"></div>
[[3bsy]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Campylobacter_jejuni Campylobacter jejuni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BSY OCA].
== References ==
 
<references/>
==Reference==
__TOC__
<ref group="xtra">PMID:018667421</ref><references group="xtra"/>
</StructureSection>
[[Category: Campylobacter jejuni]]
[[Category: UDP-N-acetylglucosamine diphosphorylase]]
[[Category: Imperiali, B.]]
[[Category: Olivier, N B.]]
[[Category: Acetyl coenzyme some]]
[[Category: Bacillosamine]]
[[Category: Campylobacter jejuni]]
[[Category: Campylobacter jejuni]]
[[Category: Hexapeptide repeat]]
[[Category: Large Structures]]
[[Category: Left-hand beta helix]]
[[Category: Imperiali B]]
[[Category: N-linked glycosylation]]
[[Category: Olivier NB]]
[[Category: Pgl]]
[[Category: Rossman fold]]
[[Category: Transferase]]
[[Category: Udp]]
Retrieved from "https://proteopedia.org/3bsy"