1y6i: Difference between revisions

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[[Image:1y6i.gif|left|200px]]<br /><applet load="1y6i" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1y6i, resolution 1.78&Aring;" />
'''Synechocystis GUN4'''<br />


==Overview==
==Synechocystis GUN4==
In plants, the accumulation of the chlorophyll precursor Mg-protoporphyrin IX (Mg-Proto) in the plastid regulates the expression of a number of nuclear genes with functions related to photosynthesis. Analysis of the plastid-to-nucleus signaling activity of Mg-Proto in Arabidopsis thaliana led to the discovery of GUN4, a novel porphyrin-binding protein that also dramatically enhances the activity of Mg-chelatase, the enzyme that synthesizes Mg-Proto. GUN4 may also play a role in both photoprotection and the cellular shuttling of tetrapyrroles. Here we report a 1.78-A resolution crystal structure of Synechocystis GUN4, in which the porphyrin-binding domain adopts a unique three dimensional fold with a "cupped hand" shape. Biophysical and biochemical analyses revealed the specific site of interaction between GUN4 and Mg-Proto and the energetic determinants for the GUN4.Mg-Proto interaction. Our data support a novel protective function for GUN4 in tetrapyrrole trafficking. The combined structural and energetic analyses presented herein form the physical-chemical basis for understanding GUN4 biological activity, including its role in the stimulation of Mg-chelatase activity, as well as in Mg-Proto retrograde signaling.
<StructureSection load='1y6i' size='340' side='right'caption='[[1y6i]], [[Resolution|resolution]] 1.78&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1y6i]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechocystis_sp._PCC_6803 Synechocystis sp. PCC 6803]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y6I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Y6I FirstGlance]. <br>
1Y6I is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Synechocystis_sp. Synechocystis sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y6I OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.78&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1y6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y6i OCA], [https://pdbe.org/1y6i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1y6i RCSB], [https://www.ebi.ac.uk/pdbsum/1y6i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1y6i ProSAT]</span></td></tr>
==Reference==
</table>
Structure of the Mg-chelatase cofactor GUN4 reveals a novel hand-shaped fold for porphyrin binding., Verdecia MA, Larkin RM, Ferrer JL, Riek R, Chory J, Noel JP, PLoS Biol. 2005 May;3(5):e151. Epub 2005 Apr 26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15884974 15884974]
== Function ==
[[Category: Single protein]]
[https://www.uniprot.org/uniprot/YC53L_SYNY3 YC53L_SYNY3]  
[[Category: Synechocystis sp.]]
== Evolutionary Conservation ==
[[Category: Chory, J.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Ferrer, J L.]]
Check<jmol>
[[Category: Larkin, R M.]]
  <jmolCheckbox>
[[Category: Noel, J P.]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/y6/1y6i_consurf.spt"</scriptWhenChecked>
[[Category: Riek, R.]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: Verdecia, M A.]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: helix-bundle]]
  </jmolCheckbox>
[[Category: porphyrin binding]]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1y6i ConSurf].
 
<div style="clear:both"></div>
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:02:13 2008''
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Synechocystis sp. PCC 6803]]
[[Category: Chory J]]
[[Category: Ferrer JL]]
[[Category: Larkin RM]]
[[Category: Noel JP]]
[[Category: Riek R]]
[[Category: Verdecia MA]]

Latest revision as of 08:55, 14 February 2024

Synechocystis GUN4

1y6i, resolution 1.78Å

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