3cwh: Difference between revisions

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[[Image:3cwh.png|left|200px]]


{{STRUCTURE_3cwh| PDB=3cwh | SCENE= }}
==D-xylose Isomerase in complex with linear product, per-deuterated xylulose==
<StructureSection load='3cwh' size='340' side='right'caption='[[3cwh]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3cwh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_rubiginosus Streptomyces rubiginosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CWH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CWH FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Neutron Diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DOD:DEUTERATED+WATER'>DOD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OH:HYDROXIDE+ION'>OH</scene>, <scene name='pdbligand=XUL:D-XYLULOSE'>XUL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cwh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cwh OCA], [https://pdbe.org/3cwh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cwh RCSB], [https://www.ebi.ac.uk/pdbsum/3cwh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cwh ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/XYLA_STRRU XYLA_STRRU] Involved in D-xylose catabolism.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cw/3cwh_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3cwh ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The time-of-flight neutron Laue technique has been used to determine the location of hydrogen atoms in the enzyme d-xylose isomerase (XI). The neutron structure of crystalline XI with bound product, d-xylulose, shows, unexpectedly, that O5 of d-xylulose is not protonated but is hydrogen-bonded to doubly protonated His54. Also, Lys289, which is neutral in native XI, is protonated (positively charged), while the catalytic water in native XI has become activated to a hydroxyl anion which is in the proximity of C1 and C2, the molecular site of isomerization of xylose. These findings impact our understanding of the reaction mechanism.


===D-xylose Isomerase in complex with linear product, per-deuterated xylulose===
Hydrogen location in stages of an enzyme-catalyzed reaction: time-of-flight neutron structure of D-xylose isomerase with bound D-xylulose.,Kovalevsky AY, Katz AK, Carrell HL, Hanson L, Mustyakimov M, Fisher SZ, Coates L, Schoenborn BP, Bunick GJ, Glusker JP, Langan P Biochemistry. 2008 Jul 22;47(29):7595-7. Epub 2008 Jun 26. PMID:18578508<ref>PMID:18578508</ref>


{{ABSTRACT_PUBMED_18578508}}
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
==About this Structure==
<div class="pdbe-citations 3cwh" style="background-color:#fffaf0;"></div>
[[3cwh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_rubiginosus Streptomyces rubiginosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CWH OCA].


==See Also==
==See Also==
*[[D-xylose isomerase|D-xylose isomerase]]
*[[D-xylose isomerase 3D structures|D-xylose isomerase 3D structures]]
 
== References ==
==Reference==
<references/>
<ref group="xtra">PMID:018578508</ref><references group="xtra"/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptomyces rubiginosus]]
[[Category: Streptomyces rubiginosus]]
[[Category: Xylose isomerase]]
[[Category: Glusker JP]]
[[Category: Glusker, J P.]]
[[Category: Kovalevsky AY]]
[[Category: Kovalevsky, A Y.]]
[[Category: Langan P]]
[[Category: Langan, P.]]
[[Category: Carbohydrate metabolism]]
[[Category: Enzyme-product complex]]
[[Category: Isomerase]]
[[Category: Magnesium]]
[[Category: Metal-binding]]
[[Category: Pentose shunt]]
[[Category: Xylose metabolism]]
Retrieved from "https://proteopedia.org/3cwh"