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[[Image:3e4b.png|left|200px]]


{{STRUCTURE_3e4b| PDB=3e4b | SCENE= }}
==Crystal structure of AlgK from Pseudomonas fluorescens WCS374r==
<StructureSection load='3e4b' size='340' side='right'caption='[[3e4b]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3e4b]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_fluorescens Pseudomonas fluorescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E4B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3E4B FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3e4b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e4b OCA], [https://pdbe.org/3e4b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3e4b RCSB], [https://www.ebi.ac.uk/pdbsum/3e4b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3e4b ProSAT]</span></td></tr>
</table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e4/3e4b_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3e4b ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The opportunistic pathogen Pseudomonas aeruginosa causes chronic biofilm infections in cystic fibrosis patients. During colonization of the lung, P. aeruginosa converts to a mucoid phenotype characterized by overproduction of the exopolysaccharide alginate. Here we show that AlgK, a protein essential for production of high molecular weight alginate, is an outer membrane lipoprotein that contributes to the correct localization of the porin AlgE. Our 2.5 A structure shows AlgK is composed of 9.5 tetratricopeptide-like repeats, and three putative sites of protein-protein interaction have been identified. Bioinformatics analysis suggests that BcsA, PgaA, and PelB, involved in the production and export of cellulose, poly-beta-1,6-N-Acetyl-D-glucosamine, and Pel exopolysaccharide, respectively, share the same topology as AlgK/E. Together, our data suggest that AlgK plays a role in the assembly of the alginate biosynthetic complex and represents the periplasmic component of a new type of outer membrane secretin that differs from canonical bacterial capsular polysaccharide secretion systems.


===Crystal structure of AlgK from Pseudomonas fluorescens WCS374r===
AlgK is a TPR-containing protein and the periplasmic component of a novel exopolysaccharide secretin.,Keiski CL, Harwich M, Jain S, Neculai AM, Yip P, Robinson H, Whitney JC, Riley L, Burrows LL, Ohman DE, Howell PL Structure. 2010 Feb 10;18(2):265-73. PMID:20159471<ref>PMID:20159471</ref>


{{ABSTRACT_PUBMED_20159471}}
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
==About this Structure==
<div class="pdbe-citations 3e4b" style="background-color:#fffaf0;"></div>
[[3e4b]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_fluorescens Pseudomonas fluorescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E4B OCA].
== References ==
 
<references/>
==Reference==
__TOC__
<ref group="xtra">PMID:020159471</ref><references group="xtra"/>
</StructureSection>
[[Category: Large Structures]]
[[Category: Pseudomonas fluorescens]]
[[Category: Pseudomonas fluorescens]]
[[Category: Burrows, L L.]]
[[Category: Burrows LL]]
[[Category: Harwich, M.]]
[[Category: Harwich M]]
[[Category: Howell, P L.]]
[[Category: Howell PL]]
[[Category: Jain, S.]]
[[Category: Jain S]]
[[Category: Keiski, C L.]]
[[Category: Keiski C-L]]
[[Category: Neculai, A M.]]
[[Category: Neculai AM]]
[[Category: Ohman, D E.]]
[[Category: Ohman DE]]
[[Category: Robinson, H.]]
[[Category: Robinson H]]
[[Category: Whitney, J C.]]
[[Category: Whitney JC]]
[[Category: Yip, P.]]
[[Category: Yip P]]
[[Category: Alginate biosynthesis]]
[[Category: Algk]]
[[Category: Protein binding]]
[[Category: Pseudomona]]
[[Category: Superhelix]]
[[Category: Tetratricopeptide repeat]]
Retrieved from "https://proteopedia.org/3e4b"