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| [[Image:1ynm.gif|left|200px]]<br /><applet load="1ynm" size="350" color="white" frame="true" align="right" spinBox="true"
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| caption="1ynm, resolution 2.65Å" />
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| '''Crystal structure of restriction endonuclease HinP1I'''<br />
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| ==Overview== | | ==Crystal structure of restriction endonuclease HinP1I== |
| HinP1I, a type II restriction endonuclease, recognizes and cleaves a palindromic tetranucleotide sequence (G/CGC) in double-stranded DNA, producing 2 nt 5' overhanging ends. Here, we report the structure of HinP1I crystallized as one protein monomer in the crystallographic asymmetric unit. HinP1I displays an elongated shape, with a conserved catalytic core domain containing an active-site motif of SDX18QXK and a putative DNA-binding domain. Without significant sequence homology, HinP1I displays striking structural similarity to MspI, an endonuclease that cleaves a similar palindromic DNA sequence (C/CGG) and binds to that sequence crystallographically as a monomer. Almost all the structural elements of MspI can be matched in HinP1I, including both the DNA recognition and catalytic elements. Examining the protein-protein interactions in the crystal lattice, HinP1I could be dimerized through two helices located on the opposite side of the protein to the active site, generating a molecule with two active sites and two DNA-binding surfaces opposite one another on the outer surfaces of the dimer. A possible functional link between this unusual dimerization mode and the tetrameric restriction enzymes is discussed.
| | <StructureSection load='1ynm' size='340' side='right'caption='[[1ynm]], [[Resolution|resolution]] 2.65Å' scene=''> |
| | == Structural highlights == |
| | <table><tr><td colspan='2'>[[1ynm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YNM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YNM FirstGlance]. <br> |
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.65Å</td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ynm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ynm OCA], [https://pdbe.org/1ynm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ynm RCSB], [https://www.ebi.ac.uk/pdbsum/1ynm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ynm ProSAT]</span></td></tr> |
| | </table> |
| | == Function == |
| | [https://www.uniprot.org/uniprot/Q5I6E6_HAEIF Q5I6E6_HAEIF] |
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| ==About this Structure== | | ==See Also== |
| 1YNM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Active as [http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YNM OCA].
| | *[[Endonuclease 3D structures|Endonuclease 3D structures]] |
| | | __TOC__ |
| ==Reference==
| | </StructureSection> |
| Structure of HinP1I endonuclease reveals a striking similarity to the monomeric restriction enzyme MspI., Yang Z, Horton JR, Maunus R, Wilson GG, Roberts RJ, Cheng X, Nucleic Acids Res. 2005 Apr 1;33(6):1892-901. Print 2005. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15805123 15805123]
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| [[Category: Haemophilus influenzae]] | | [[Category: Haemophilus influenzae]] |
| [[Category: Single protein]] | | [[Category: Large Structures]] |
| [[Category: Type II site-specific deoxyribonuclease]]
| | [[Category: Cheng X]] |
| [[Category: Cheng, X.]] | | [[Category: Horton JR]] |
| [[Category: Horton, J R.]] | | [[Category: Maunus R]] |
| [[Category: Maunus, R.]] | | [[Category: Roberts RJ]] |
| [[Category: Roberts, R J.]] | | [[Category: Wilson GG]] |
| [[Category: Wilson, G G.]] | | [[Category: Yang Z]] |
| [[Category: Yang, Z.]] | |
| [[Category: dimerizaton]]
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| [[Category: restriction endonuclease]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:07:14 2008''
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