4irm: Difference between revisions
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New page: '''Unreleased structure''' The entry 4irm is ON HOLD Authors: Kanteev, M., Adir, N. Description: Crystal structure of mntc r116a mutant exhibits flexibility in the c-terminal domain |
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The entry | ==Crystal structure of mntc r116a mutant exhibits flexibility in the c-terminal domain== | ||
<StructureSection load='4irm' size='340' side='right'caption='[[4irm]], [[Resolution|resolution]] 3.50Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4irm]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechocystis_sp._PCC_6803 Synechocystis sp. PCC 6803]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3v63 3v63]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IRM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IRM FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4irm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4irm OCA], [https://pdbe.org/4irm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4irm RCSB], [https://www.ebi.ac.uk/pdbsum/4irm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4irm ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q79EF9_SYNY3 Q79EF9_SYNY3] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The cyanobacterium Synechocystis sp. PCC 6803 imports Mn2+ ions via MntCAB, an ABC transport system that is expressed at submicromolar Mn2+ concentrations. The structures of the wild type (WT) and a site-directed mutant of the MntC solute-binding protein have been determined at 2.7 and 3.5 A resolution, respectively. The WT structure is significantly improved over the previously determined structure (PDB entry 1xvl), showing improved Mn2+ binding site parameters, disulfide bonds in all three monomers and ions bound to the protein surface, revealing the role of Zn2+ ions in the crystallization liquor. The structure of MntC reveals that the active site is surrounded by neutral-to-positive electrostatic potential and is dominated by a network of polar interactions centred around Arg116. The mutation of this residue to alanine was shown to destabilize loops in the entrance to the metal-ion binding site and suggests a possible role in MntC function. | |||
Arginine 116 stabilizes the entrance to the metal ion-binding site of the MntC protein.,Kanteev M, Adir N Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Mar 1;69(Pt 3):237-42., doi: 10.1107/S174430911300153X. Epub 2013 Feb 22. PMID:23519795<ref>PMID:23519795</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4irm" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Synechocystis sp. PCC 6803]] | |||
[[Category: Adir N]] | |||
[[Category: Kanteev M]] | |||
Latest revision as of 15:29, 20 September 2023
Crystal structure of mntc r116a mutant exhibits flexibility in the c-terminal domain
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