4i8c: Difference between revisions
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==X-ray structure of NikA in complex with Ni-(L-His)2== | |||
<StructureSection load='4i8c' size='340' side='right'caption='[[4i8c]], [[Resolution|resolution]] 2.50Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4i8c]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4I8C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4I8C FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.503Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HIS:HISTIDINE'>HIS</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4i8c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4i8c OCA], [https://pdbe.org/4i8c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4i8c RCSB], [https://www.ebi.ac.uk/pdbsum/4i8c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4i8c ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/NIKA_ECOLI NIKA_ECOLI] Involved in a nickel transport system, probably represents the nickel binder. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The ABC-type importer NikABCDE mediates nickel acquisition in Escherichia coli. The periplasmic nickel-binding component NikA has a folding similar to that of the peptide transporter OppA and does not bind free nickel. Instead, we showed that the metal is tetra-coordinated by an organic tri-dentate molecule and His416. Conversely, it has been recently reported that NikA binds Ni-((L)-His)(2) and that addition of histidine increases the rate of nickel uptake in vivo. Here, we report the structure of NikA/Ni-((L)-His)(2) and show that histidine binding differs from peptide binding in OppA. The structure also confirms the central role of His416 in nickel binding. | |||
The binding mode of Ni-((L)-His)(2) in NikA revealed by X-ray crystallography.,Lebrette H, Iannello M, Fontecilla-Camps JC, Cavazza C J Inorg Biochem. 2012 Dec 23;121C:16-18. doi: 10.1016/j.jinorgbio.2012.12.010. PMID:23314594<ref>PMID:23314594</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4i8c" style="background-color:#fffaf0;"></div> | |||
== | ==See Also== | ||
[[ | *[[ABC transporter 3D structures|ABC transporter 3D structures]] | ||
[[Category: | == References == | ||
[[Category: Cavazza | <references/> | ||
[[Category: Fontecilla-Camps | __TOC__ | ||
[[Category: Iannello | </StructureSection> | ||
[[Category: Lebrette | [[Category: Escherichia coli K-12]] | ||
[[Category: Large Structures]] | |||
[[Category: Cavazza C]] | |||
[[Category: Fontecilla-Camps JC]] | |||
[[Category: Iannello M]] | |||
[[Category: Lebrette H]] | |||
Latest revision as of 15:17, 20 September 2023
X-ray structure of NikA in complex with Ni-(L-His)2
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