3zkr: Difference between revisions
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==X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) in complex with bromoform== | |||
<StructureSection load='3zkr' size='340' side='right'caption='[[3zkr]], [[Resolution|resolution]] 3.65Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3zkr]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Dickeya_chrysanthemi Dickeya chrysanthemi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZKR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZKR FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.649Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MBR:TRIBROMOMETHANE'>MBR</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zkr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zkr OCA], [https://pdbe.org/3zkr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zkr RCSB], [https://www.ebi.ac.uk/pdbsum/3zkr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zkr ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/ELIC_DICCH ELIC_DICCH] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Pentameric ligand-gated ion channels (pLGICs), such as nicotinic acetylcholine, glycine, gamma-aminobutyric acid GABAA/C receptors, and the Gloeobacter violaceus ligand-gated ion channel (GLIC), are receptors that contain multiple allosteric binding sites for a variety of therapeutics, including general anesthetics. Here, we report the x-ray crystal structure of the Erwinia chrysanthemi ligand-gated ion channel (ELIC) in complex with a derivative of chloroform, which reveals important features of anesthetic recognition, involving multiple binding at three different sites. One site is located in the channel pore and equates with a noncompetitive inhibitor site found in many pLGICs. A second transmembrane site is novel and is located in the lower part of the transmembrane domain, at an interface formed between adjacent subunits. A third site is also novel and is located in the extracellular domain in a hydrophobic pocket between the beta7-beta10 strands. Together, these results extend our understanding of pLGIC modulation and reveal several specific binding interactions that may contribute to modulator recognition, further substantiating a multisite model of allosteric modulation in this family of ion channels. | |||
Multisite Binding of a General Anesthetic to the Prokaryotic Pentameric Erwinia chrysanthemi Ligand-gated Ion Channel (ELIC).,Spurny R, Billen B, Howard RJ, Brams M, Debaveye S, Price KL, Weston DA, Strelkov SV, Tytgat J, Bertrand S, Bertrand D, Lummis SC, Ulens C J Biol Chem. 2013 Mar 22;288(12):8355-64. doi: 10.1074/jbc.M112.424507. Epub 2013, Jan 30. PMID:23364792<ref>PMID:23364792</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 3zkr" style="background-color:#fffaf0;"></div> | |||
== | ==See Also== | ||
[[ | *[[Ion channels 3D structures|Ion channels 3D structures]] | ||
[[Category: | == References == | ||
[[Category: Bertrand | <references/> | ||
[[Category: Bertrand | __TOC__ | ||
[[Category: Billen | </StructureSection> | ||
[[Category: Brams | [[Category: Dickeya chrysanthemi]] | ||
[[Category: Debaveye | [[Category: Large Structures]] | ||
[[Category: Howard | [[Category: Bertrand D]] | ||
[[Category: Lummis | [[Category: Bertrand S]] | ||
[[Category: Price | [[Category: Billen B]] | ||
[[Category: Spurny | [[Category: Brams M]] | ||
[[Category: Strelkov | [[Category: Debaveye S]] | ||
[[Category: Tytgat | [[Category: Howard RJ]] | ||
[[Category: Ulens | [[Category: Lummis SCR]] | ||
[[Category: Weston | [[Category: Price KL]] | ||
[[Category: Spurny R]] | |||
[[Category: Strelkov SV]] | |||
[[Category: Tytgat J]] | |||
[[Category: Ulens C]] | |||
[[Category: Weston DA]] | |||
Latest revision as of 10:40, 9 May 2024
X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) in complex with bromoform
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