4hx9: Difference between revisions
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==Designed Phosphodeoxyribosyltransferase== | |||
<StructureSection load='4hx9' size='340' side='right'caption='[[4hx9]], [[Resolution|resolution]] 2.68Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4hx9]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactobacillus_leichmannii Lactobacillus leichmannii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HX9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HX9 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.68Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hx9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hx9 OCA], [https://pdbe.org/4hx9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hx9 RCSB], [https://www.ebi.ac.uk/pdbsum/4hx9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hx9 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/NTD_LACLE NTD_LACLE] Catalyzes the cleavage of the glycosidic bond of 2'-deoxyribonucleosides and the transfer of the deoxyribosyl moiety to an acceptor purine or pyrimidine base.<ref>PMID:7797550</ref> <ref>PMID:8805514</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
A large number of nucleoside analogs and 2'- deoxynucleoside triphosphates (dNTPs) have been synthesized in order to interfere with DNA metabolism. However, in vivo the concentration and phosphorylation of these analogues are key limiting factors. In this context, we designed enzymes to switch nucleobases attached to a deoxyribose monophosphate. Active chimeras were made from two distantly related enzymes: a nucleoside deoxyribosyltransferase (NDT) from lactobacilli and a 5'-monophosphate-2'-deoxyribonucleoside hydrolase (Rcl) from rat. Then, their unprecedented activity was further extended to deoxyribose triphosphate and, in vitro biosyntheses could be successfully performed with several base analogues. These new enzymes provide new tools to synthesize dNTPs analogs and to deliver them into cells. | |||
Phosphodeoxyribosyltransferases: designed enzymes for deoxyribonucleotide synthesis.,Kaminski PA, Labesse G J Biol Chem. 2013 Jan 16. PMID:23325804<ref>PMID:23325804</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== | <div class="pdbe-citations 4hx9" style="background-color:#fffaf0;"></div> | ||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Lactobacillus leichmannii]] | [[Category: Lactobacillus leichmannii]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Kaminski | [[Category: Kaminski PA]] | ||
[[Category: Labesse | [[Category: Labesse G]] | ||
Latest revision as of 15:12, 20 September 2023
Designed Phosphodeoxyribosyltransferase
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