2m4k: Difference between revisions
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New page: '''Unreleased structure''' The entry 2m4k is ON HOLD Authors: Papouskova, V., Novacek, J., Kaderavek, P., Zidek, L., Rabatinova, A., Sanderova, H., Krasny, L., Sklenar, V. Description:... |
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The | ==Solution structure of the delta subunit of RNA polymerase from Bacillus subtilis== | ||
<StructureSection load='2m4k' size='340' side='right'caption='[[2m4k]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2m4k]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M4K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2M4K FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2m4k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m4k OCA], [https://pdbe.org/2m4k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2m4k RCSB], [https://www.ebi.ac.uk/pdbsum/2m4k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2m4k ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/RPOE_BACSU RPOE_BACSU] Participates in both the initiation and recycling phases of transcription. In the presence of the delta subunit, RNAP displays an increased specificity of transcription, a decreased affinity for nucleic acids, and an increased efficiency of RNA synthesis because of enhanced recycling. May function in sigma factor switching. It displaces RNA bound to RNA polymerase in a binary complex. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The partially disordered delta subunit of RNA polymerase was studied by various NMR techniques. The structure of the well-folded N-terminal domain was determined based on inter-proton distances in NOESY spectra. The obtained structural model was compared to the previously determined structure of a truncated construct (lacking the C-terminal domain). Only marginal differences were identified, thus indicating that the first structural model was not significantly compromised by the absence of the C-terminal domain. Various (15) N relaxation experiments were employed to describe the flexibility of both domains. The relaxation data revealed that the C-terminal domain is more flexible, but its flexibility is not uniform. By using paramagnetic labels, transient contacts of the C-terminal tail with the N-terminal domain and with itself were identified. A propensity of the C-terminal domain to form beta-type structures was obtained by chemical shift analysis. Comparison with the paramagnetic relaxation enhancement indicated a well-balanced interplay of repulsive and attractive electrostatic interactions governing the conformational behavior of the C-terminal domain. The results showed that the delta subunit consists of a well-ordered N-terminal domain and a flexible C-terminal domain that exhibits a complex hierarchy of partial ordering. | |||
Structural Study of the Partially Disordered Full-Length delta Subunit of RNA Polymerase from Bacillus subtilis.,Papouskova V, Kaderavek P, Otrusinova O, Rabatinova A, Sanderova H, Novacek J, Krasny L, Sklenar V, Zidek L Chembiochem. 2013 Sep 23;14(14):1772-9. doi: 10.1002/cbic.201300226. Epub 2013, Jul 18. PMID:23868186<ref>PMID:23868186</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2m4k" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[RNA polymerase 3D structures|RNA polymerase 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Bacillus subtilis subsp. subtilis str. 168]] | |||
[[Category: Large Structures]] | |||
[[Category: Kaderavek P]] | |||
[[Category: Krasny L]] | |||
[[Category: Novacek J]] | |||
[[Category: Papouskova V]] | |||
[[Category: Rabatinova A]] | |||
[[Category: Sanderova H]] | |||
[[Category: Sklenar V]] | |||
[[Category: Zidek L]] | |||
