4j4m: Difference between revisions
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New page: '''Unreleased structure''' The entry 4j4m is ON HOLD Authors: Chou, T.L., Wu, C.H., Huang, K.F., Wang, A.H. Description: Crystal structure of TM-1, a Trimeresurus mucrosquamatus venom ... |
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The | ==Crystal structure of TM-1, a Trimeresurus mucrosquamatus venom metalloproteinase== | ||
<StructureSection load='4j4m' size='340' side='right'caption='[[4j4m]], [[Resolution|resolution]] 1.80Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4j4m]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Protobothrops_mucrosquamatus Protobothrops mucrosquamatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J4M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4J4M FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4j4m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j4m OCA], [https://pdbe.org/4j4m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4j4m RCSB], [https://www.ebi.ac.uk/pdbsum/4j4m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4j4m ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/VM1T1_PROMU VM1T1_PROMU] Potent fibrinogenolytic protease which cleaves mainly the Aalpha (FGA) and Bbeta (FGB) chains of fibrinogen and slightly the gamma chain (FGG) (PubMed:7488093, PubMed:8193588). Shows preference for substrates having a moderate-size and hydrophobic residue at the P1' position. Preferentially cleaves Ala-|-Leu and Tyr-|-Leu bonds (PubMed:23732127). Is more susceptible to tripeptide inhibitors than TM-3 (AC O57413) (PubMed:9703966).<ref>PMID:23732127</ref> <ref>PMID:7488093</ref> <ref>PMID:8193588</ref> <ref>PMID:9703966</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The crystal structure of TM-1, a P-I class snake-venom metalloproteinase (SVMP) from the Trimeresurus mucrosquamatus venom, was determined at 1.8-A resolution. The structure exhibits the typical feature of SVMPs and is stabilized by three disulfide linkages. The active site shows a deep S1' substrate-binding pocket limited by the non-conserved Pro174 at the bottom. Further comparisons with other SVMPs suggest that the deep S1' site of TM-1 correlates with its high inhibition sensitivity to the endogenous tripeptide inhibitors. Proteolytic specificity analysis revealed that TM-1 prefers substrates having a moderate-size and hydrophobic residue at the P1' position, consistent with our structural observation. | |||
Crystal structure of a Trimeresurus mucrosquamatus venom metalloproteinase providing new insights into the inhibition by endogenous tripeptide inhibitors.,Chou TL, Wu CH, Huang KF, Wang AH Toxicon. 2013 Sep;71:140-6. doi: 10.1016/j.toxicon.2013.05.009. Epub 2013 May 31. PMID:23732127<ref>PMID:23732127</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4j4m" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Protobothrops mucrosquamatus]] | |||
[[Category: Chou TL]] | |||
[[Category: Huang KF]] | |||
[[Category: Wang AH]] | |||
[[Category: Wu CH]] | |||
Latest revision as of 11:00, 6 November 2024
Crystal structure of TM-1, a Trimeresurus mucrosquamatus venom metalloproteinase
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