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| [[Image:208d.gif|left|200px]]<br /><applet load="208d" size="350" color="white" frame="true" align="right" spinBox="true"
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| caption="208d, resolution 2.050Å" />
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| '''HIGH-RESOLUTION STRUCTURE OF A DNA HELIX FORMING (C.G)*G BASE TRIPLETS'''<br />
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| ==Overview== | | ==HIGH-RESOLUTION STRUCTURE OF A DNA HELIX FORMING (C.G)*G BASE TRIPLETS== |
| Triple helices result from interaction between single- and double-stranded nucleic acids. Their formation is a possible mechanism for recombination of homologous gene sequences in nature and provides, inter alia, a basis for artificial control of gene activity. Triple-helix motifs have been extensively studied by a variety of techniques, but few high-resolution structural data are available. The only triplet structures characterized so far by X-ray diffraction were in protein-DNA complexes studied at about 3 A resolution. We report here the X-ray analysis of a DNA nonamer, d(GCGAATTCG), to a resolution of 2.05 A, in which the extended crystal structure contains (C.G)*G triplets as a fragment of triple helix. The guanosine-containing chains are in a parallel orientation. This arrangement is a necessary feature of models for homologous recombination which results ultimately in replacement of one length of DNA by another of similar sequence. The present-structure agrees with many published predictions of triplex organization, and provides an accurate representation of an element that allows sequence-specific association between single- and double-stranded nucleic acids.
| | <StructureSection load='208d' size='340' side='right'caption='[[208d]], [[Resolution|resolution]] 2.05Å' scene=''> |
| | | == Structural highlights == |
| ==About this Structure== | | <table><tr><td colspan='2'>[[208d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=208D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=208D FirstGlance]. <br> |
| 208D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=208D OCA].
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05Å</td></tr> |
| | | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| ==Reference==
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=208d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=208d OCA], [https://pdbe.org/208d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=208d RCSB], [https://www.ebi.ac.uk/pdbsum/208d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=208d ProSAT]</span></td></tr> |
| High-resolution structure of a DNA helix forming (C.G)*G base triplets., Van Meervelt L, Vlieghe D, Dautant A, Gallois B, Precigoux G, Kennard O, Nature. 1995 Apr 20;374(6524):742-4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7715732 7715732]
| | </table> |
| [[Category: Protein complex]] | | __TOC__ |
| [[Category: Dautant, A.]] | | </StructureSection> |
| [[Category: Gallois, B.]] | | [[Category: Large Structures]] |
| [[Category: Kennard, O.]] | | [[Category: Dautant A]] |
| [[Category: Meervelt, L Van.]] | | [[Category: Gallois B]] |
| [[Category: Precigoux, G.]] | | [[Category: Kennard O]] |
| [[Category: Vlieghe, D.]] | | [[Category: Precigoux G]] |
| [[Category: MG]]
| | [[Category: Van Meervelt L]] |
| [[Category: b-dna]]
| | [[Category: Vlieghe D]] |
| [[Category: base triplet]]
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| [[Category: double helix]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:20:51 2008''
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