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| [[Image:243d.gif|left|200px]]<br /><applet load="243d" size="350" color="white" frame="true" align="right" spinBox="true"
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| caption="243d, resolution 1.900Å" />
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| '''STRUCTURE OF THE DNA OCTANUCLEOTIDE D(ACGTACGT)2'''<br />
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| ==Overview== | | ==STRUCTURE OF THE DNA OCTANUCLEOTIDE D(ACGTACGT)2== |
| d(ACGTACGT), C(78)H(84)N(30)O(32)P(7).20H(2)O, M(r) (DNA) = 2170, tetragonal, P4(3)2(1)2 (No 96), a = 42.845 (1), b = 42.845(1), c = 24.804 (1) A, V = 45532.5 (2) A(3), z = 8,lambda(MoKalpha) = 0.71069 A, micro (MoKalpha) = 0.10 mm(-1), T = 295 K, R = 0.18 for 1994 unique reflections between 5.0 and 1.9 A resolution. The self-complementary octanucleotide d(ACGTACGT)(2) has been crystallized and its structure determined to a resolution of 1.9 A. The asymmetric unit consists of a single strand of octamer with 20 water molecules. It is only the second example of an octanucleotide having terminal A.T base pairs whose structure has been determined by X-ray crystallography. The sequence adopts the modified A-type conformation found for all octanucleotide duplexes studied to date with the helix bent by approximately 15 degrees and an average tilt angle of 0 degrees. Unusually the data collection was carried out using a 3 kW molybdenum sealed-tube source. The conformational details are discussed in comparison with other closely related sequences.
| | <StructureSection load='243d' size='340' side='right'caption='[[243d]], [[Resolution|resolution]] 1.90Å' scene=''> |
| | | == Structural highlights == |
| ==About this Structure== | | <table><tr><td colspan='2'>[[243d]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=243D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=243D FirstGlance]. <br> |
| 243D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=243D OCA].
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> |
| | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=243d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=243d OCA], [https://pdbe.org/243d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=243d RCSB], [https://www.ebi.ac.uk/pdbsum/243d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=243d ProSAT]</span></td></tr> |
| ==Reference==
| | </table> |
| Structure of the DNA octanucleotide d(ACGTACGT)2., Wilcock DJ, Adams A, Cardin CJ, Wakelin LP, Acta Crystallogr D Biol Crystallogr. 1996 May 1;52(Pt 3):481-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15299669 15299669]
| | __TOC__ |
| [[Category: Protein complex]] | | </StructureSection> |
| [[Category: Adams, A.]] | | [[Category: Large Structures]] |
| [[Category: Cardin, C J.]] | | [[Category: Adams A]] |
| [[Category: Wakelin, L P.G.]] | | [[Category: Cardin CJ]] |
| [[Category: Wilcock, D J.]] | | [[Category: Wakelin LPG]] |
| [[Category: a-dna]]
| | [[Category: Wilcock DJ]] |
| [[Category: double helix]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:21:34 2008''
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