2lys: Difference between revisions
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==NOE-based 3D structure of the monomeric partially-folded intermediate of CylR2 at 257K (-16 Celsius degrees)== | |||
<StructureSection load='2lys' size='340' side='right'caption='[[2lys]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2lys]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LYS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LYS FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lys FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lys OCA], [https://pdbe.org/2lys PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lys RCSB], [https://www.ebi.ac.uk/pdbsum/2lys PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lys ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q8VL32_ENTFL Q8VL32_ENTFL] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Protein folding and unfolding are crucial for a range of biological phenomena and human diseases. Defining the structural properties of the involved transient species is therefore of prime interest. Using a combination of cold denaturation with NMR spectroscopy, we reveal detailed insight into the unfolding of the homodimeric repressor protein CylR2. Seven three-dimensional structures of CylR2 at temperatures from 25 degrees C to -16 degrees C reveal a progressive dissociation of the dimeric protein into a native-like monomeric intermediate followed by transition into a highly dynamic, partially folded state. The core of the partially folded state seems critical for biological function and misfolding. | |||
Cold denaturation of a protein dimer monitored at atomic resolution.,Jaremko M, Jaremko L, Kim HY, Cho MK, Schwieters CD, Giller K, Becker S, Zweckstetter M Nat Chem Biol. 2013 Feb 10. doi: 10.1038/nchembio.1181. PMID:23396077<ref>PMID:23396077</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
== | </div> | ||
<div class="pdbe-citations 2lys" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Enterococcus faecalis]] | [[Category: Enterococcus faecalis]] | ||
[[Category: Becker | [[Category: Large Structures]] | ||
[[Category: Cho | [[Category: Becker S]] | ||
[[Category: Giller | [[Category: Cho M]] | ||
[[Category: Jaremko | [[Category: Giller K]] | ||
[[Category: Jaremko | [[Category: Jaremko L]] | ||
[[Category: Kim | [[Category: Jaremko M]] | ||
[[Category: Schwieters | [[Category: Kim H]] | ||
[[Category: Zweckstetter | [[Category: Schwieters CD]] | ||
[[Category: Zweckstetter M]] | |||
Latest revision as of 05:55, 15 May 2024
NOE-based 3D structure of the monomeric partially-folded intermediate of CylR2 at 257K (-16 Celsius degrees)
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