3fyh: Difference between revisions

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[[Image:3fyh.png|left|200px]]


{{STRUCTURE_3fyh| PDB=3fyh | SCENE= }}
==Recombinase in complex with ADP and metatungstate==
<StructureSection load='3fyh' size='340' side='right'caption='[[3fyh]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3fyh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanococcus_voltae Methanococcus voltae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FYH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FYH FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=W:TUNGSTEN+ION'>W</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fyh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fyh OCA], [https://pdbe.org/3fyh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fyh RCSB], [https://www.ebi.ac.uk/pdbsum/3fyh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fyh ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RADA_METVO RADA_METVO] Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules (By similarity).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fy/3fyh_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fyh ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Archaeal RadAs are close homologues of eukaryal Rad51s ( approximately 40% sequence identities). These recombinases promote a hallmark strand exchange process between homologous single-stranded and double-stranded DNA substrates. This DNA-repairing function also plays a key role in cancer cells' resistance to chemo- and radiotherapy. Inhibition of the strand exchange process may render cancer cells more susceptible to therapeutic treatment. We found that metatungstate is a potent inhibitor of RadA from Methanococcus voltae. The tungsten cluster binds RadA in the axial DNA-binding groove. This polyanionic species appears to inhibit RadA by locking the protein in its inactive conformation.


===Recombinase in complex with ADP and metatungstate===
Crystal structure of an archaeal Rad51 homologue in complex with a metatungstate inhibitor.,Li Y, He Y, Luo Y Biochemistry. 2009 Jul 28;48(29):6805-10. PMID:19555119<ref>PMID:19555119</ref>


{{ABSTRACT_PUBMED_19555119}}
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3fyh" style="background-color:#fffaf0;"></div>


==About this Structure==
==See Also==
[[3fyh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Methanococcus_voltae Methanococcus voltae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FYH OCA].
*[[Resolvase 3D structures|Resolvase 3D structures]]
 
== References ==
==Reference==
<references/>
<ref group="xtra">PMID:019555119</ref><references group="xtra"/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Methanococcus voltae]]
[[Category: Methanococcus voltae]]
[[Category: He, Y.]]
[[Category: He Y]]
[[Category: Li, Y.]]
[[Category: Li Y]]
[[Category: Luo, Y.]]
[[Category: Luo Y]]
[[Category: Atp-binding]]
[[Category: Atpase]]
[[Category: Dna damage]]
[[Category: Dna recombination]]
[[Category: Dna-binding]]
[[Category: Inhibitor]]
[[Category: Nucleotide-binding]]
[[Category: Rad51]]
[[Category: Rada]]
[[Category: Rada-inhibitor complex]]
[[Category: Reca]]
[[Category: Recombinase]]
[[Category: Recombination]]
Retrieved from "https://proteopedia.org/3fyh"