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[[Image:2acw.gif|left|200px]]<br /><applet load="2acw" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2acw, resolution 2.6&Aring;" />
'''Crystal Structure of Medicago truncatula UGT71G1 complexed with UDP-glucose'''<br />


==Overview==
==Crystal Structure of Medicago truncatula UGT71G1 complexed with UDP-glucose==
Glycosylation is a ubiquitous reaction controlling the bioactivity and storage of plant natural products. Glycosylation of small molecules is catalyzed by a superfamily of glycosyltransferases (GTs) in most plant species studied to date. We present crystal structures of the UDP flavonoid/triterpene GT UGT71G1 from Medicago truncatula bound to UDP or UDP-glucose. The structures reveal the key residues involved in the recognition of donor substrate and, by comparison with other GT structures, suggest His-22 as the catalytic base and Asp-121 as a key residue that may assist deprotonation of the acceptor by forming an electron transfer chain with the catalytic base. Mutagenesis confirmed the roles of these key residues in donor substrate binding and enzyme activity. Our results provide an initial structural basis for understanding the complex substrate specificity and regiospecificity underlying the glycosylation of plant natural products and other small molecules. This information will direct future attempts to engineer bioactive compounds in crop plants to improve plant, animal, and human health and to facilitate the rational design of GTs to improve the storage and stability of novel engineered bioactive compounds.
<StructureSection load='2acw' size='340' side='right'caption='[[2acw]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[2acw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Medicago_truncatula Medicago truncatula]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ACW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ACW FirstGlance]. <br>
2ACW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Medicago_truncatula Medicago truncatula] with <scene name='pdbligand=UPG:'>UPG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ACW OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UPG:URIDINE-5-DIPHOSPHATE-GLUCOSE'>UPG</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2acw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2acw OCA], [https://pdbe.org/2acw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2acw RCSB], [https://www.ebi.ac.uk/pdbsum/2acw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2acw ProSAT]</span></td></tr>
Crystal structures of a multifunctional triterpene/flavonoid glycosyltransferase from Medicago truncatula., Shao H, He X, Achnine L, Blount JW, Dixon RA, Wang X, Plant Cell. 2005 Nov;17(11):3141-54. Epub 2005 Oct 7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16214900 16214900]
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q5IFH7_MEDTR Q5IFH7_MEDTR]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ac/2acw_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2acw ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Medicago truncatula]]
[[Category: Medicago truncatula]]
[[Category: Single protein]]
[[Category: Achnine L]]
[[Category: Achnine, L.]]
[[Category: Blount JW]]
[[Category: Blount, J W.]]
[[Category: Dixon RA]]
[[Category: Dixon, R A.]]
[[Category: He X]]
[[Category: He, X.]]
[[Category: Shao H]]
[[Category: Shao, H.]]
[[Category: Wang X]]
[[Category: Wang, X.]]
[[Category: UPG]]
[[Category: glycosyltransferase]]
[[Category: udp-glucose]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:26:05 2008''
Retrieved from "https://proteopedia.org/2acw"