3gfj: Difference between revisions

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[[Image:3gfj.png|left|200px]]


{{STRUCTURE_3gfj| PDB=3gfj | SCENE= }}
==Crystal structure of the ST1710 mutant (R89A) protein==
<StructureSection load='3gfj' size='340' side='right'caption='[[3gfj]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3gfj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfurisphaera_tokodaii Sulfurisphaera tokodaii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GFJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GFJ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gfj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gfj OCA], [https://pdbe.org/3gfj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gfj RCSB], [https://www.ebi.ac.uk/pdbsum/3gfj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gfj ProSAT], [https://www.topsan.org/Proteins/RSGI/3gfj TOPSAN]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q96ZY1_SULTO Q96ZY1_SULTO]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gf/3gfj_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3gfj ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
ST1710, a member of the multiple antibiotic resistance regulator (MarR) family of regulatory proteins in bacteria and archaea, plays important roles in development of antibiotic resistance, a global health problem. Here, we present the crystal structure of ST1710 from Sulfolobus tokodaii strain 7 complexed with salicylate, a well-known inhibitor of MarR proteins and the ST1710 complex with its promoter DNA, refined to 1.8 and 2.10 A resolutions, respectively. The ST1710-DNA complex shares the topology of apo-ST1710 and MarR proteins, with each subunit containing a winged helix-turn-helix (wHtH) DNA binding motif. Significantly large conformational changes occurred upon DNA binding and in each of the dimeric monomers in the asymmetric unit of the ST1710-DNA complex. Conserved wHtH loop residues interacting with the bound DNA and mutagenic analysis indicated that R89, R90 and K91 were important for DNA recognition. Significantly, the bound DNA exhibited a new binding mechanism.


===Crystal structure of the ST1710 mutant (R89A) protein===
ST1710-DNA complex crystal structure reveals the DNA binding mechanism of the MarR family of regulators.,Kumarevel T, Tanaka T, Umehara T, Yokoyama S Nucleic Acids Res. 2009 Aug;37(14):4723-35. Epub 2009 Jun 9. PMID:19509310<ref>PMID:19509310</ref>


{{ABSTRACT_PUBMED_19509310}}
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
==About this Structure==
<div class="pdbe-citations 3gfj" style="background-color:#fffaf0;"></div>
[[3gfj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_tokodaii Sulfolobus tokodaii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GFJ OCA].
== References ==
 
<references/>
==Reference==
__TOC__
<ref group="xtra">PMID:019509310</ref><ref group="xtra">PMID:017933554</ref><references group="xtra"/>
</StructureSection>
[[Category: Sulfolobus tokodaii]]
[[Category: Large Structures]]
[[Category: Kumarevel, T.]]
[[Category: Sulfurisphaera tokodaii]]
[[Category: Tanaka, T.]]
[[Category: Kumarevel T]]
[[Category: Yokoyama, S.]]
[[Category: Tanaka T]]
[[Category: Dna-binding]]
[[Category: Yokoyama S]]
[[Category: Marr]]
[[Category: National project on protein structural and functional analyse]]
[[Category: Nppsfa]]
[[Category: Riken structural genomics/proteomics initiative]]
[[Category: Rsgi]]
[[Category: St1710]]
[[Category: Structural genomic]]
[[Category: Transcription]]
[[Category: Transcription regulation]]
[[Category: Transcription regulator]]

Latest revision as of 15:39, 1 November 2023

Crystal structure of the ST1710 mutant (R89A) protein

3gfj, resolution 2.20Å

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