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[[Image:2avw.gif|left|200px]]<br /><applet load="2avw" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2avw, resolution 2.0&Aring;" />
'''Crystal structure of monoclinic form of streptococcus Mac-1'''<br />


==Overview==
==Crystal structure of monoclinic form of streptococcus Mac-1==
Group A Streptococcus secretes cysteine proteases named Mac-1 and Mac-2 that mediate host immune evasion by targeting both IgG and Fc receptors. Here, we report the crystal structures of Mac-1 and its catalytically inactive C94A mutant in two different crystal forms. Despite the lack of sequence homology, Mac-1 adopts the canonical papain fold. Alanine mutations at the active site confirmed the critical residues involved in a papain-like catalytic mechanism. Mac-1 forms a symmetric dimer in both crystal forms and displays the unique dimer interface among papain superfamily members. Mutations at the dimer interface resulted in a significant reduction in IgG binding and catalysis, suggesting that the dimer contributes to both IgG specificity and enzyme cooperativity. A tunnel observed at the dimer interface constitutes a target for designing potential Mac-1-specific antimicrobial agents. The structures also offer insight into the functional difference between Mac-1 and Mac-2.
<StructureSection load='2avw' size='340' side='right'caption='[[2avw]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[2avw]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pyogenes_MGAS5005 Streptococcus pyogenes MGAS5005]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AVW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AVW FirstGlance]. <br>
2AVW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_pyogenes_mgas315 Streptococcus pyogenes mgas315] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AVW OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2avw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2avw OCA], [https://pdbe.org/2avw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2avw RCSB], [https://www.ebi.ac.uk/pdbsum/2avw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2avw ProSAT]</span></td></tr>
Crystal structure of group A streptococcus Mac-1: insight into dimer-mediated specificity for recognition of human IgG., Agniswamy J, Nagiec MJ, Liu M, Schuck P, Musser JM, Sun PD, Structure. 2006 Feb;14(2):225-35. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16472742 16472742]
</table>
[[Category: Single protein]]
== Function ==
[[Category: Streptococcus pyogenes mgas315]]
[https://www.uniprot.org/uniprot/Q7DAM2_STRP1 Q7DAM2_STRP1]
[[Category: Agniswamy, J.]]
== Evolutionary Conservation ==
[[Category: Liu, M.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Musser, J M.]]
Check<jmol>
[[Category: Nagiec, M J.]]
  <jmolCheckbox>
[[Category: Schuck, P.]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/av/2avw_consurf.spt"</scriptWhenChecked>
[[Category: Sun, P D.]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: GOL]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: SO4]]
  </jmolCheckbox>
[[Category: mac-1]]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2avw ConSurf].
 
<div style="clear:both"></div>
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:31:38 2008''
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptococcus pyogenes MGAS5005]]
[[Category: Agniswamy J]]
[[Category: Liu M]]
[[Category: Musser JM]]
[[Category: Nagiec MJ]]
[[Category: Schuck P]]
[[Category: Sun PD]]

Latest revision as of 09:14, 14 February 2024

Crystal structure of monoclinic form of streptococcus Mac-1

2avw, resolution 2.00Å

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