Barnase: Difference between revisions

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[[Image:1bni.png|left|200px|thumb|Crystal Structure of Barnase [[1bni]]]]
<StructureSection load='1brn' size='350' side='right' scene='44/442736/Cv/2' caption='Barnase complex with tetranucleotide (PDB code [[1brn]])'>
{{STRUCTURE_1bni|  PDB=1bni  | SIZE=400| SCENE=Barnase/Cv/1 |right|CAPTION=3 monomers of barnase [[1bni]] }}


== Function ==


'''Barnase''' (BA, BActerial RiboNucleASE) is a bacterial [[ribonuclease]] synthesized and secreted by the bacterium ''Bacillus amyloliquefaciens''. <ref>PMID:8110767</ref> The inhibitor barstar (BST) binds to it very tightly and prevents it from digestion of the cell’s RNA.


== Relevance ==


BA structure was used extensively for studying protein folding.


== Structural highlights ==


 
BA hydrolyzes RNA at GpN sites where the <scene name='44/442736/Cv/4'>active site positively charged Lys and Arg coordinate the RNA phosphate group</scene> (Lys and Arg are in magenta).
 
 
 
 
 
 
 
 
 
 
 
 
'''Barnase''' (BA, BActerial RiboNucleASE) is a bacterial ribonuclease synthesized and secreted by the bacterium ''Bacillus amyloliquefaciens''.  The inhibitor barstar (BST) binds to it very tightly and prevents it from digestion of the cell’s RNA.
 
__NOTOC__


== 3D Structures of Barnase ==
== 3D Structures of Barnase ==
[[Barnase 3D structures]]


Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
</StructureSection>
 
[[3kch]], [[2f4y]] – BA cross-linked<br />
[[2f56]] - BA cross-linked + urea<br />
[[2f5w]] - BA cross-linked + thiourea<br />
[[2f5m]] - BA cross-linked + Bromo-ethanol<br />
[[1fw7]], [[1bnr]] – BA - NMR<br />
[[2kf3]] – BA (mutant) – NMR<br />
[[2kf4]], [[1b2x]], [[1a2p]], [[1bni]], [[1bnj]] – BA<br />
[[1yvs]] – BA domain swapped<br />
[[1b20]], [[1b21]], [[1b2z]], [[1bne]], [[1bnf]], [[1bng]], [[1brh]], [[1bri]], [[1brj]], [[1brk]], [[1bns]], [[1brg]], [[1bsa]], [[1bsb]], [[1bsc]], [[1bsd]], [[1bse]], [[1ban]], [[1bao]] – BA (mutant)<br />
[[2kf5]], [[2kf6]], [[1brn]], [[1rnb]] – BA + polynucleotide
 
===BA + BST===
 
[[3da7]] - BA (mutant) + BST<br />
[[2za4]], [[1brs]], [[1bgs]] – BA + BST<br />
[[1x1u]], [[1x1w]], [[1x1x]], [[1x1y]], [[1b27]], [[1b2u]] – BA + BST (mutant)<br />
[[1b3s]], [[1b2s]] - BA (mutant) + BST (mutant)<br />
 
[[2c4b]] – BA/MCOEETI (mutant)


== References ==
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Latest revision as of 14:37, 20 February 2022

Barnase complex with tetranucleotide (PDB code 1brn)

Drag the structure with the mouse to rotate

References

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Jaime Prilusky

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