2b3t: Difference between revisions

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[[Image:2b3t.gif|left|200px]]<br /><applet load="2b3t" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2b3t, resolution 3.100&Aring;" />
'''Molecular basis for bacterial class 1 release factor methylation by PrmC'''<br />


==Overview==
==Structure of complex between E. coli translation termination factor RF1 and the PrmC methyltransferase==
Class I release factors bind to ribosomes in response to stop codons and trigger peptidyl-tRNA hydrolysis at the P site. Prokaryotic and eukaryotic RFs share one motif: a GGQ tripeptide positioned in a loop at the end of a stem region that interacts with the ribosomal peptidyl transferase center. The glutamine side chain of this motif is specifically methylated in both prokaryotes and eukaryotes. Methylation in E. coli is due to PrmC and results in strong stimulation of peptide chain release. We have solved the crystal structure of the complex between E. coli RF1 and PrmC bound to the methyl donor product AdoHCy. Both the GGQ domain (domain 3) and the central region (domains 2 and 4) of RF1 interact with PrmC. Structural and mutagenic data indicate a compact conformation of RF1 that is unlike its conformation when it is bound to the ribosome but is similar to the crystal structure of the protein alone.
<StructureSection load='2b3t' size='340' side='right'caption='[[2b3t]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[2b3t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B3T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B3T FirstGlance]. <br>
2B3T is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=SAH:'>SAH</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B3T OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b3t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b3t OCA], [https://pdbe.org/2b3t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b3t RCSB], [https://www.ebi.ac.uk/pdbsum/2b3t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b3t ProSAT]</span></td></tr>
Molecular basis for bacterial class I release factor methylation by PrmC., Graille M, Heurgue-Hamard V, Champ S, Mora L, Scrima N, Ulryck N, van Tilbeurgh H, Buckingham RH, Mol Cell. 2005 Dec 22;20(6):917-27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16364916 16364916]
</table>
== Function ==
[https://www.uniprot.org/uniprot/PRMC_ECOLI PRMC_ECOLI] Methylates the class 1 translation termination release factors RF1/PrfA and RF2/PrfB on the glutamine residue of the universally conserved GGQ motif, i.e. on 'Gln-235' in RF1 and on 'Gln-252' in RF2.<ref>PMID:11805295</ref> <ref>PMID:11847124</ref> <ref>PMID:16364916</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b3/2b3t_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2b3t ConSurf].
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Protein complex]]
[[Category: Large Structures]]
[[Category: Buckingham, R H.]]
[[Category: Buckingham RH]]
[[Category: Champ, S.]]
[[Category: Champ S]]
[[Category: Graille, M.]]
[[Category: Graille M]]
[[Category: Heurgue-Hamard, V.]]
[[Category: Heurgue-Hamard V]]
[[Category: Mora, L.]]
[[Category: Mora L]]
[[Category: Scrima, N.]]
[[Category: Scrima N]]
[[Category: Tilbeurgh, H van.]]
[[Category: Ulryck N]]
[[Category: Ulryck, N.]]
[[Category: Van Tilbeurgh H]]
[[Category: SAH]]
[[Category: release factor; translation termination; methylation; conformational changes]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:33:52 2008''
Retrieved from "https://proteopedia.org/2b3t"