1o7t: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| (19 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
== | ==Metal nanoclusters bound to the Ferric Binding Protein from Neisseria gonorrhoeae.== | ||
Transferrins transport Fe3+ and other metal ions in mononuclear-binding | <StructureSection load='1o7t' size='340' side='right'caption='[[1o7t]], [[Resolution|resolution]] 1.65Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1o7t]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Neisseria_gonorrhoeae Neisseria gonorrhoeae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1O7T FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HF3:SMALLEST+HF-OXO-PHOSPHATE+CLUSTER+HF3'>HF3</scene>, <scene name='pdbligand=HF5:HF+OXO+CLUSTER+HF5'>HF5</scene>, <scene name='pdbligand=PHF:HF-OXO-PHOSPHATE+CLUSTER+PHF'>PHF</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1o7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o7t OCA], [https://pdbe.org/1o7t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1o7t RCSB], [https://www.ebi.ac.uk/pdbsum/1o7t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1o7t ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q50964_NEIGO Q50964_NEIGO] | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o7/1o7t_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1o7t ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Transferrins transport Fe3+ and other metal ions in mononuclear-binding sites. We present the first evidence that a member of the transferrin superfamily is able to recognize multi-nuclear oxo-metal clusters, small mineral fragments that are the most abundant forms of many metals in the environment. We show that the ferric ion-binding protein from Neisseria gonorrhoeae (nFbp) readily binds clusters of Fe3+, Ti4+, Zr4+ or Hf4+ in solution. The 1.7 A resolution crystal structure of Hf-nFbp reveals three distinct types of clusters in an open, positively charged cleft between two hinged protein domains. A di-tyrosyl cluster nucleation motif (Tyr195-Tyr196) is situated at the bottom of this cleft and binds either a trinuclear oxo-Hf cluster, which is capped by phosphate, or a pentanuclear cluster, which in turn can be capped with phosphate. This first high-resolution structure of a protein-mineral interface suggests a novel metal-uptake mechanism and provides a model for protein-mediated mineralization/dissimilation, which plays a critical role in geochemical processes. | |||
A novel protein-mineral interface.,Alexeev D, Zhu H, Guo M, Zhong W, Hunter DJ, Yang W, Campopiano DJ, Sadler PJ Nat Struct Biol. 2003 Apr;10(4):297-302. PMID:12598891<ref>PMID:12598891</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
<div class="pdbe-citations 1o7t" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Ferric-binding protein|Ferric-binding protein]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Neisseria gonorrhoeae]] | [[Category: Neisseria gonorrhoeae]] | ||
[[Category: Alexeev D]] | |||
[[Category: Alexeev | [[Category: Campopiano DJ]] | ||
[[Category: Campopiano | [[Category: Guo M]] | ||
[[Category: Guo | [[Category: Hunter DJB]] | ||
[[Category: Hunter | [[Category: Sadler PJ]] | ||
[[Category: Sadler | [[Category: Yang W]] | ||
[[Category: Yang | [[Category: Zhong W]] | ||
[[Category: Zhong | [[Category: Zu H]] | ||
[[Category: Zu | |||
