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{{STRUCTURE_2hlv|  PDB=2hlv  |  SCENE=  }}
===Bovine Odorant Binding Protein deswapped triple mutant===
{{ABSTRACT_PUBMED_18269920}}


==About this Structure==
==Bovine Odorant Binding Protein deswapped triple mutant==
[[2hlv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HLV OCA].  
<StructureSection load='2hlv' size='340' side='right'caption='[[2hlv]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2hlv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HLV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HLV FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=LIK:3,6-BIS(METHYLENE)DECANOIC+ACID'>LIK</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hlv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hlv OCA], [https://pdbe.org/2hlv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hlv RCSB], [https://www.ebi.ac.uk/pdbsum/2hlv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hlv ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/OBP_BOVIN OBP_BOVIN] This protein binds a wide variety of chemical odorants.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hl/2hlv_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hlv ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The X-ray structure of bovine Odorant Binding Protein (bOBP) revealed its association as a domain swapped dimer. bOBP, devoid of any cysteines, contrasts with other mammalian OBPs, which are monomeric and possess at least one disulfide bridge. We have produced a mutant of bOBP in which a glycine residue was inserted after position 121. This mutation yielded a monomeric bOBP-121Gly+ in which domain swapping has been reverted. Here, we have subsequently introduced two mutations, Trp64Cys and His155Cys, in view to stabilize the putative monomer with a disulfide bridge. We have determined the crystal structure of this triple mutant at 1.65 A resolution. The mutant protein is monomeric, stabilized by a disulfide bridge between Trp64Cys and His155Cys, with a backbone superimposable to that of native bOBP, with the exception of the hinge and of the 10 residues at the C-terminus. bOBP triple mutant binds 1-amino-anthracene, 1-octen-3-ol (bOBP co-purified ligand) and other ligands with microM Kd values comparable to those of the swapped dimer.
 
Deswapping bovine odorant binding protein.,Ramoni R, Spinelli S, Grolli S, Conti V, Merli E, Cambillau C, Tegoni M Biochim Biophys Acta. 2008 Apr;1784(4):651-7. Epub 2008 Jan 29. PMID:18269920<ref>PMID:18269920</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2hlv" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Odorant binding protein|Odorant binding protein]]
*[[Odorant binding protein 3D structures|Odorant binding protein 3D structures]]
 
== References ==
==Reference==
<references/>
<ref group="xtra">PMID:018269920</ref><references group="xtra"/>
__TOC__
</StructureSection>
[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: Cambillau, C.]]
[[Category: Large Structures]]
[[Category: Spinelli, S.]]
[[Category: Cambillau C]]
[[Category: Tegoni, M.]]
[[Category: Spinelli S]]
[[Category: Domain swapping]]
[[Category: Tegoni M]]
[[Category: Odorant binding protein]]
[[Category: Transport protein]]
Retrieved from "https://proteopedia.org/2hlv"