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[[Image:2cnz.gif|left|200px]]<br /><applet load="2cnz" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2cnz, resolution 1.80&Aring;" />
'''SALMONELLA ENTERICA SAFA PILIN IN COMPLEX WITH A 19-RESIDUE SAFA NTE PEPTIDE (V13A MUTANT)'''<br />


==Overview==
==Salmonella enterica SafA pilin in complex with a 19-residue SafA Nte peptide (V13A mutant)==
Gram-negative pathogens commonly use the chaperone-usher pathway to assemble adhesive multisubunit fibers on their surface. In the periplasm, subunits are stabilized by a chaperone that donates a beta strand to complement the subunits' truncated immunoglobulin-like fold. Pilus assembly proceeds through a "donor-strand exchange" (DSE) mechanism whereby this complementary beta strand is replaced by the N-terminal extension (Nte) of an incoming pilus subunit. Using X-ray crystallography and real-time electrospray ionization mass spectrometry (ESI-MS), we demonstrate that DSE requires the formation of a transient ternary complex between the chaperone-subunit complex and the Nte of the next subunit to be assembled. The process is crucially dependent on an initiation site (the P5 pocket) needed to recruit the incoming Nte. The data also suggest a capping reaction displacing DSE toward product formation. These results support a zip-in-zip-out mechanism for DSE and a catalytic role for the usher, the molecular platform at which pili are assembled.
<StructureSection load='2cnz' size='340' side='right'caption='[[2cnz]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2cnz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CNZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CNZ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cnz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cnz OCA], [https://pdbe.org/2cnz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cnz RCSB], [https://www.ebi.ac.uk/pdbsum/2cnz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cnz ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q8ZRK4_SALTY Q8ZRK4_SALTY]


==About this Structure==
==See Also==
2CNZ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CNZ OCA].
*[[Pilin 3D structures|Pilin 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Donor-strand exchange in chaperone-assisted pilus assembly proceeds through a concerted beta strand displacement mechanism., Remaut H, Rose RJ, Hannan TJ, Hultgren SJ, Radford SE, Ashcroft AE, Waksman G, Mol Cell. 2006 Jun 23;22(6):831-42. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16793551 16793551]
[[Category: Large Structures]]
[[Category: Protein complex]]
[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
[[Category: Salmonella typhimurium]]
[[Category: Ashcroft AE]]
[[Category: Ashcroft, A E.]]
[[Category: Hannan TJ]]
[[Category: Hannan, T J.]]
[[Category: Hultgren SJ]]
[[Category: Hultgren, S J.]]
[[Category: Radford SE]]
[[Category: Radford, S E.]]
[[Category: Remaut H]]
[[Category: Remaut, H.]]
[[Category: Rose RJ]]
[[Category: Rose, R J.]]
[[Category: Waksman G]]
[[Category: Waksman, G.]]
[[Category: fibril protein]]
[[Category: fold complementation]]
[[Category: pilus subunit]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:50:39 2008''

Latest revision as of 06:39, 1 May 2024

Salmonella enterica SafA pilin in complex with a 19-residue SafA Nte peptide (V13A mutant)

2cnz, resolution 1.80Å

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