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{{STRUCTURE_3jtr|  PDB=3jtr  |  SCENE=  }}
===Mutations in Cephalosporin Acylase Affecting Stability and Autoproteolysis===
{{ABSTRACT_PUBMED_19800869}}


==About this Structure==
==Mutations in Cephalosporin Acylase Affecting Stability and Autoproteolysis==
[[3jtr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_sp. Pseudomonas sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JTR OCA].  
<StructureSection load='3jtr' size='340' side='right'caption='[[3jtr]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3jtr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_sp._GK16 Pseudomonas sp. GK16]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JTR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3JTR FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3jtr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jtr OCA], [https://pdbe.org/3jtr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3jtr RCSB], [https://www.ebi.ac.uk/pdbsum/3jtr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3jtr ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A4ZVL3_PSEU7 A4ZVL3_PSEU7]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jt/3jtr_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3jtr ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Cephalosporin acylase (CA), a member of the N-terminal nucleophile hydrolase family, is activated through two steps of intramolecular autoproteolysis, the first mediated by a serine residue, and the second by a glutamate, which releases the pro-segment and produces an active enzyme. In this study, we have determined the crystal structures of mutants which could affect primary or secondary auto-cleavage and of sequential intermediates of a slow-processing mutant at 2.0-2.5A resolutions. The pro-segments of the mutants undergo dynamic conformational changes during activation and adopt surprisingly different loop conformations from one another. However, the autoproteolytic site was found to form a catalytically competent conformation with a solvent water molecule, which was essentially conserved in the CA mutants.


==Reference==
Structural features of cephalosporin acylase reveal the basis of autocatalytic activation.,Cho KJ, Kim JK, Lee JH, Shin HJ, Park SS, Kim KH Biochem Biophys Res Commun. 2009 Dec 11;390(2):342-8. Epub 2009 Oct 2. PMID:19800869<ref>PMID:19800869</ref>
<ref group="xtra">PMID:019800869</ref><references group="xtra"/><references/>
 
[[Category: Glutaryl-7-aminocephalosporanic-acid acylase]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Pseudomonas sp.]]
</div>
[[Category: Cho, K J.]]
<div class="pdbe-citations 3jtr" style="background-color:#fffaf0;"></div>
[[Category: Kim, J K.]]
 
[[Category: Kim, K H.]]
==See Also==
[[Category: Lee, J H.]]
*[[Cephalosporin acylase 3D structures|Cephalosporin acylase 3D structures]]
[[Category: Park, S S.]]
== References ==
[[Category: Shin, H J.]]
<references/>
[[Category: Autoproteolysis]]
__TOC__
[[Category: Cephalosporin acylase]]
</StructureSection>
[[Category: Hydrolase]]
[[Category: Large Structures]]
[[Category: Pseudomonas sp. GK16]]
[[Category: Cho KJ]]
[[Category: Kim JK]]
[[Category: Kim KH]]
[[Category: Lee JH]]
[[Category: Park SS]]
[[Category: Shin HJ]]
Retrieved from "https://proteopedia.org/3jtr"