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{{STRUCTURE_3zkr|  PDB=3zkr  |  SCENE=  }}
===X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) in complex with bromoform===
{{ABSTRACT_PUBMED_23364792}}


==About this Structure==
==X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) in complex with bromoform==
[[3zkr]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZKR OCA].  
<StructureSection load='3zkr' size='340' side='right'caption='[[3zkr]], [[Resolution|resolution]] 3.65&Aring;' scene=''>
[[Category: Erwinia chrysanthemi]]
== Structural highlights ==
[[Category: Bertrand, D.]]
<table><tr><td colspan='2'>[[3zkr]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Dickeya_chrysanthemi Dickeya chrysanthemi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZKR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZKR FirstGlance]. <br>
[[Category: Bertrand, S.]]
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.649&#8491;</td></tr>
[[Category: Billen, B.]]
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MBR:TRIBROMOMETHANE'>MBR</scene></td></tr>
[[Category: Brams, M.]]
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zkr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zkr OCA], [https://pdbe.org/3zkr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zkr RCSB], [https://www.ebi.ac.uk/pdbsum/3zkr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zkr ProSAT]</span></td></tr>
[[Category: Debaveye, S.]]
</table>
[[Category: Howard, R J.]]
== Function ==
[[Category: Lummis, S C.R.]]
[https://www.uniprot.org/uniprot/ELIC_DICCH ELIC_DICCH]
[[Category: Price, K L.]]
<div style="background-color:#fffaf0;">
[[Category: Spurny, R.]]
== Publication Abstract from PubMed ==
[[Category: Strelkov, S V.]]
Pentameric ligand-gated ion channels (pLGICs), such as nicotinic acetylcholine, glycine, gamma-aminobutyric acid GABAA/C receptors, and the Gloeobacter violaceus ligand-gated ion channel (GLIC), are receptors that contain multiple allosteric binding sites for a variety of therapeutics, including general anesthetics. Here, we report the x-ray crystal structure of the Erwinia chrysanthemi ligand-gated ion channel (ELIC) in complex with a derivative of chloroform, which reveals important features of anesthetic recognition, involving multiple binding at three different sites. One site is located in the channel pore and equates with a noncompetitive inhibitor site found in many pLGICs. A second transmembrane site is novel and is located in the lower part of the transmembrane domain, at an interface formed between adjacent subunits. A third site is also novel and is located in the extracellular domain in a hydrophobic pocket between the beta7-beta10 strands. Together, these results extend our understanding of pLGIC modulation and reveal several specific binding interactions that may contribute to modulator recognition, further substantiating a multisite model of allosteric modulation in this family of ion channels.
[[Category: Tytgat, J.]]
 
[[Category: Ulens, C.]]
Multisite Binding of a General Anesthetic to the Prokaryotic Pentameric Erwinia chrysanthemi Ligand-gated Ion Channel (ELIC).,Spurny R, Billen B, Howard RJ, Brams M, Debaveye S, Price KL, Weston DA, Strelkov SV, Tytgat J, Bertrand S, Bertrand D, Lummis SC, Ulens C J Biol Chem. 2013 Mar 22;288(12):8355-64. doi: 10.1074/jbc.M112.424507. Epub 2013, Jan 30. PMID:23364792<ref>PMID:23364792</ref>
[[Category: Weston, D A.]]
 
[[Category: Cys-loop receptor]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Gaba-a receptor]]
</div>
[[Category: General anesthetic]]
<div class="pdbe-citations 3zkr" style="background-color:#fffaf0;"></div>
[[Category: Transport protein]]
 
==See Also==
*[[Ion channels 3D structures|Ion channels 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Dickeya chrysanthemi]]
[[Category: Large Structures]]
[[Category: Bertrand D]]
[[Category: Bertrand S]]
[[Category: Billen B]]
[[Category: Brams M]]
[[Category: Debaveye S]]
[[Category: Howard RJ]]
[[Category: Lummis SCR]]
[[Category: Price KL]]
[[Category: Spurny R]]
[[Category: Strelkov SV]]
[[Category: Tytgat J]]
[[Category: Ulens C]]
[[Category: Weston DA]]
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