4jox: Difference between revisions

Latest revision as of 12:05, 1 March 2024

Crystal structure of cry34Ab1 protein at 2.15 A resolution

Structural highlights

4jox is a 1 chain structure with sequence from Bacillus thuringiensis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.15Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

C34AB_BACTU Component of a binary insecticidal toxin active on western corn rootworm (WCR, Diabrotica virgifera subsp. virgifera Le Conte) and probably also on northern corn rootworm (D.barberi). Both proteins are required for maximal toxicity. The larval midgut epithelium is probably the primary target (PubMed:11433280, PubMed:11872461). This protein alone is also active against southern corn rootworm (Diabrotica undecimpunctata howardi Barber), but its activity is synergized by its Cry35Ab1 partner (PubMed:12076012). The 2 proteins individually and together form ion channels; channels made in the presence of the 2 proteins have higher conductance (PubMed:15379574). Significantly ameliorates the binding of Cry35Ab1 to WCR third instar midgut brush border membrane vesicles. Binding of this protein by itself could not be studied for technical reasons (PubMed:23308139).^[1] ^[2] ^[3] ^[4] ^[5]

References

↑ Moellenbeck DJ, Peters ML, Bing JW, Rouse JR, Higgins LS, Sims L, Nevshemal T, Marshall L, Ellis RT, Bystrak PG, Lang BA, Stewart JL, Kouba K, Sondag V, Gustafson V, Nour K, Xu D, Swenson J, Zhang J, Czapla T, Schwab G, Jayne S, Stockhoff BA, Narva K, Schnepf HE, Stelman SJ, Poutre C, Koziel M, Duck N. Insecticidal proteins from Bacillus thuringiensis protect corn from corn rootworms. Nat Biotechnol. 2001 Jul;19(7):668-72. doi: 10.1038/90282. PMID:11433280 doi:https://dx.doi.org/10.1038/90282
↑ Ellis RT, Stockhoff BA, Stamp L, Schnepf HE, Schwab GE, Knuth M, Russell J, Cardineau GA, Narva KE. Novel Bacillus thuringiensis binary insecticidal crystal proteins active on western corn rootworm, Diabrotica virgifera virgifera LeConte. Appl Environ Microbiol. 2002 Mar;68(3):1137-45. PMID:11872461
↑ Herman RA, Scherer PN, Young DL, Mihaliak CA, Meade T, Woodsworth AT, Stockhoff BA, Narva KE. Binary insecticidal crystal protein from Bacillus thuringiensis, strain PS149B1: effects of individual protein components and mixtures in laboratory bioassays. J Econ Entomol. 2002 Jun;95(3):635-9. doi: 10.1603/0022-0493-95.3.635. PMID:12076012 doi:https://dx.doi.org/10.1603/0022-0493-95.3.635
↑ Masson L, Schwab G, Mazza A, Brousseau R, Potvin L, Schwartz JL. A novel Bacillus thuringiensis (PS149B1) containing a Cry34Ab1/Cry35Ab1 binary toxin specific for the western corn rootworm Diabrotica virgifera virgifera LeConte forms ion channels in lipid membranes. Biochemistry. 2004 Sep 28;43(38):12349-57. doi: 10.1021/bi048946z. PMID:15379574 doi:https://dx.doi.org/10.1021/bi048946z
↑ Li H, Olson M, Lin G, Hey T, Tan SY, Narva KE. Bacillus thuringiensis Cry34Ab1/Cry35Ab1 interactions with western corn rootworm midgut membrane binding sites. PLoS One. 2013;8(1):e53079. doi: 10.1371/journal.pone.0053079. Epub 2013 Jan 4. PMID:23308139 doi:https://dx.doi.org/10.1371/journal.pone.0053079

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OCA

[1] Moellenbeck DJ, Peters ML, Bing JW, Rouse JR, Higgins LS, Sims L, Nevshemal T, Marshall L, Ellis RT, Bystrak PG, Lang BA, Stewart JL, Kouba K, Sondag V, Gustafson V, Nour K, Xu D, Swenson J, Zhang J, Czapla T, Schwab G, Jayne S, Stockhoff BA, Narva K, Schnepf HE, Stelman SJ, Poutre C, Koziel M, Duck N. Insecticidal proteins from Bacillus thuringiensis protect corn from corn rootworms. Nat Biotechnol. 2001 Jul;19(7):668-72. doi: 10.1038/90282. PMID:11433280 doi:https://dx.doi.org/10.1038/90282

[2] Ellis RT, Stockhoff BA, Stamp L, Schnepf HE, Schwab GE, Knuth M, Russell J, Cardineau GA, Narva KE. Novel Bacillus thuringiensis binary insecticidal crystal proteins active on western corn rootworm, Diabrotica virgifera virgifera LeConte. Appl Environ Microbiol. 2002 Mar;68(3):1137-45. PMID:11872461

[3] Herman RA, Scherer PN, Young DL, Mihaliak CA, Meade T, Woodsworth AT, Stockhoff BA, Narva KE. Binary insecticidal crystal protein from Bacillus thuringiensis, strain PS149B1: effects of individual protein components and mixtures in laboratory bioassays. J Econ Entomol. 2002 Jun;95(3):635-9. doi: 10.1603/0022-0493-95.3.635. PMID:12076012 doi:https://dx.doi.org/10.1603/0022-0493-95.3.635

[4] Masson L, Schwab G, Mazza A, Brousseau R, Potvin L, Schwartz JL. A novel Bacillus thuringiensis (PS149B1) containing a Cry34Ab1/Cry35Ab1 binary toxin specific for the western corn rootworm Diabrotica virgifera virgifera LeConte forms ion channels in lipid membranes. Biochemistry. 2004 Sep 28;43(38):12349-57. doi: 10.1021/bi048946z. PMID:15379574 doi:https://dx.doi.org/10.1021/bi048946z

[5] Li H, Olson M, Lin G, Hey T, Tan SY, Narva KE. Bacillus thuringiensis Cry34Ab1/Cry35Ab1 interactions with western corn rootworm midgut membrane binding sites. PLoS One. 2013;8(1):e53079. doi: 10.1371/journal.pone.0053079. Epub 2013 Jan 4. PMID:23308139 doi:https://dx.doi.org/10.1371/journal.pone.0053079

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 4jox is ON HOLD  until Paper Publication
+==Crystal structure of cry34Ab1 protein at 2.15 A resolution==
+<StructureSection load='4jox' size='340' side='right'caption='[[4jox]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
-Authors: Yang, C., Pflugrath, J.W., Kelker, M.S., Evan, S.L., Narva, K.E.
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4jox]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_thuringiensis Bacillus thuringiensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JOX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4JOX FirstGlance]. <br>
-Description: Crystal structure of cry34Ab1 protein at 2.15 A resolution
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4jox FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jox OCA], [https://pdbe.org/4jox PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4jox RCSB], [https://www.ebi.ac.uk/pdbsum/4jox PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4jox ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/C34AB_BACTU C34AB_BACTU] Component of a binary insecticidal toxin active on western corn rootworm (WCR, Diabrotica virgifera subsp. virgifera Le Conte) and probably also on northern corn rootworm (D.barberi). Both proteins are required for maximal toxicity. The larval midgut epithelium is probably the primary target (PubMed:11433280, PubMed:11872461). This protein alone is also active against southern corn rootworm (Diabrotica undecimpunctata howardi Barber), but its activity is synergized by its Cry35Ab1 partner (PubMed:12076012). The 2 proteins individually and together form ion channels; channels made in the presence of the 2 proteins have higher conductance (PubMed:15379574). Significantly ameliorates the binding of Cry35Ab1 to WCR third instar midgut brush border membrane vesicles. Binding of this protein by itself could not be studied for technical reasons (PubMed:23308139).<ref>PMID:11433280</ref> <ref>PMID:11872461</ref> <ref>PMID:12076012</ref> <ref>PMID:15379574</ref> <ref>PMID:23308139</ref>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Bacillus thuringiensis]]
+[[Category: Large Structures]]
+[[Category: Evan SL]]
+[[Category: Kelker MS]]
+[[Category: Narva KE]]
+[[Category: Pflugrath JW]]
+[[Category: Yang C]]

4jox: Difference between revisions

Latest revision as of 12:05, 1 March 2024

Crystal structure of cry34Ab1 protein at 2.15 A resolution

Structural highlights

Function

References

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