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{{STRUCTURE_3m5b|  PDB=3m5b  |  SCENE=  }}
===Crystal structure of the BTB domain from FAZF/ZBTB32===
{{ABSTRACT_PUBMED_20493880}}


==Function==
==Crystal structure of the BTB domain from FAZF/ZBTB32==
[[http://www.uniprot.org/uniprot/ZBT32_HUMAN ZBT32_HUMAN]] DNA-binding protein that binds to the to a 5'-TGTACAGTGT-3' core sequence. May function as a transcriptional transactivator and transcriptional repressor. Probably exerts its repressor effect by preventing GATA3 from binding to DNA. May play a role in regulating the differentiation and activation of helper T-cells (By similarity).<ref>PMID:10572087</ref>
<StructureSection load='3m5b' size='340' side='right'caption='[[3m5b]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3m5b]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M5B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3M5B FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3m5b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m5b OCA], [https://pdbe.org/3m5b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3m5b RCSB], [https://www.ebi.ac.uk/pdbsum/3m5b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3m5b ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ZBT32_HUMAN ZBT32_HUMAN] DNA-binding protein that binds to the to a 5'-TGTACAGTGT-3' core sequence. May function as a transcriptional transactivator and transcriptional repressor. Probably exerts its repressor effect by preventing GATA3 from binding to DNA. May play a role in regulating the differentiation and activation of helper T-cells (By similarity).<ref>PMID:10572087</ref>  
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m5/3m5b_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3m5b ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The BTB domain is a widely distributed protein-protein interaction motif that is often found at the N-terminus of zinc finger transcription factors. Previous crystal structures of BTB domains have revealed tightly interwound homodimers, with the N-terminus from one chain forming a two-stranded anti-parallel beta-sheet with a strand from the other chain. We have solved the crystal structures of the BTB domains from Fanconi anemia zinc finger (FAZF) and Miz1 (Myc-interacting zinc finger 1) to resolutions of 2.0 A and 2.6 A, respectively. Unlike previous examples of BTB domain structures, the FAZF BTB domain is a nonswapped dimer, with each N-terminal beta-strand associated with its own chain. As a result, the dimerization interface in the FAZF BTB domain is about half as large as in the domain-swapped dimers. The Miz1 BTB domain resembles a typical swapped BTB dimer, although it has a shorter N-terminus that is not able to form the interchain sheet. Using cysteine cross-linking, we confirmed that the promyelocytic leukemia zinc finger (PLZF) BTB dimer is strand exchanged in solution, while the FAZF BTB dimer is not. A phylogenic tree of the BTB fold based on both sequence and structural features shows that the common ancestor of the BTB domain in BTB-ZF (bric a brac, tramtrack, broad-complex zinc finger) proteins was a domain-swapped dimer. The differences in the N-termini seen in the FAZF and Miz1 BTB domains appear to be more recent developments in the structural evolution of the domain.


==About this Structure==
Insights into strand exchange in BTB domain dimers from the crystal structures of FAZF and Miz1.,Stogios PJ, Cuesta-Seijo JA, Chen L, Pomroy NC, Prive GG J Mol Biol. 2010 Jul 30;400(5):983-97. Epub 2010 May 21. PMID:20493880<ref>PMID:20493880</ref>
[[3m5b]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M5B OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
<ref group="xtra">PMID:020493880</ref><references group="xtra"/><references/>
</div>
<div class="pdbe-citations 3m5b" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Pomroy, N C.]]
[[Category: Large Structures]]
[[Category: Prive, G G.]]
[[Category: Pomroy NC]]
[[Category: Stogios, P J.]]
[[Category: Prive GG]]
[[Category: Alpha/beta protein]]
[[Category: Stogios PJ]]
[[Category: Btb domain]]
[[Category: Btb/poz domain]]
[[Category: Dna binding protein]]
[[Category: Dna-binding]]
[[Category: Fancc-interacting protein]]
[[Category: Fanconi anemia zinc finger protein]]
[[Category: Faxf]]
[[Category: Fazf]]
[[Category: Metal-binding]]
[[Category: Nucleus]]
[[Category: Poz domain]]
[[Category: Protein-protein interaction domain]]
[[Category: Repressor]]
[[Category: Rog]]
[[Category: Testis zinc finger protein]]
[[Category: Transcription]]
[[Category: Transcription regulation]]
[[Category: Transcription regulator]]
[[Category: Tzfp]]
[[Category: Zbtb32]]
[[Category: Zinc finger and btb domain-containing protein 32]]
[[Category: Zinc-finger]]
[[Category: Zinc-finger protein]]
[[Category: Znf538]]
Retrieved from "https://proteopedia.org/3m5b"